影响细菌种群(太岁组织)结构关键因素的研究  

Study on the Key Factors Affecting the Bacterial Population Structure of Taisui Tissue

作　　者：王朝江[1] 李慧[1] 王世清[1] 高春燕[1] 李聪 张洋 刘方[2] Wang Chaojiang;Li Hui;Wang Shiqing;Gao Chunyan;Li Cong;Zhang Yang;Liu Fang(Institute of Commercial Crop of Hebei Academy of Agriculture and Forestry Science,Shijiazhuang 050051 China;College of Life Sciences,Nankai University,Tianjin 300071,China)

机构地区：[1]河北省农林科学院经济作物研究所,河北石家庄050051 [2]南开大学生命科学学院,天津300071

出　　处：《南开大学学报（自然科学版）》2022年第3期73-80,共8页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：河北省农林科学院农业科技创新工程项目(2-05-03)。

摘　　要：探索了太岁取样部位和DNA提取前处理2个因素对细菌菌群结构的影响,并获知真实的优势菌群信息.对表里部位重复取样样品,施加水浸提、热溶解与微孔过滤组合成的3种前处理,提取的DNA用Miseq平台测序,挖掘出共有、占比高、有最高丰度OTU支撑的菌群.发现太岁中细菌种类丰富,大部分是杂菌,由表至内,多数类群不明原因消失,多样性和丰度降低,假单胞菌属(Pseudomonas)丰度显著升高;其与不动杆菌属(Acinetobacter)、根瘤菌属(Rhizobium)和鞘脂菌属(Sphingobium)为共有优势属;3种前处理方法获得的菌群结构近似,以内部组织直接热溶解过膜法为最优,其假单胞菌属占比达48.32%.In this researches,the effects of two factors were explored,the sampling site of Taisui and preDNA extraction on the structure of bacterial flora.The sometimes,the information on the dominant community of Taisui was obtained and the duplicate sample groups in different parts were extracted using different pretreatments by water immersion,thermal dissolution and micro-pore filtration.By determining the bacterium community structure,the Miseq platform analyzed and excavated the dominant bacteria with common,high proportion and maximum abundance supported by OTU.The results showed that there were many kinds of bacteria in Taisui and most of them are miscellaneous bacteria.Numerous bacteria disappeared for unknown reasons from outer to inner and diversity and abundance decreased.Moreover,the abundance of Pseudomonas increased significantly,and Pseudomonas was the common dominance with Acinetobacter、Rhizobium and Sphingobium.All the three pretreatment methods could obtain the same dominant group,however,the direct thermal dissolution of the internal tissue was optimal,by which Pseudomonas had a high proportion for 48.32%.

关 键 词：太岁 细菌 组织前处理 DNA提取 菌群结构 

分 类 号：Q93[生物学—微生物学]