急性果糖摄入对健康青年人的血液抗氧化能力和肝酶的影响  

Short-term effects of acute fructose intake on blood antioxidant capacity and liver enzymes in healthy young adults

作　　者：聂秀玲[1] 张晶[1] 宋林凌 赵伟[1] 成晓翠 孙蓓[1] Nie Xiuling;Zhang Jing;Song Linling;Zhao Wei;Cheng Xiaocui;Sun Pei(Department of Gout,National Health Commission Key Laboratory of Hormones and Development(Tianjin Medical University),Tianjin Key Laboratory of Metabolic Diseases,Tianjin Medical University Chu Hsien-I Memorial Hospital&Tianjin Institute of Endocrinology,Tianjin 300134,China)

机构地区：[1]国家卫生健康委员会激素与发育重点实验室(天津医科大学),天津市代谢性疾病重点实验室,天津医科大学朱宪彝纪念医院&内分泌研究所痛风科,天津300134

出　　处：《中国医师进修杂志》2022年第7期587-593,共7页Chinese Journal of Postgraduates of Medicine

摘　　要：目的探讨一次果糖摄入对健康青年人血液抗氧化能力和肝酶的短期影响。方法2019年1—6月招募青年健康受试者64例,按随机数字表法分为75 g葡萄糖组、25 g果糖组、50 g果糖组和75 g果糖组,每组16例。各组受试者按分组分别服用相应量的葡萄糖或果糖。服糖前及服糖后30、60、120和180 min检测丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、谷胱甘肽过氧化物酶(GPX)、过氧化物歧化酶(SOD)、C-Jun氨基末端激酶(JNK)、丙二醛(MDA)、8-羟基脱氧鸟嘌呤(8-OHdG)水平,计算服糖后30、60、120和180 min ALT、AST和LDH较服糖前的变化值。结果50 g果糖组1例、75 g果糖组2例、75 g葡萄糖组1例因服糖后不良反应退出,最终75 g葡萄糖组15例、25 g果糖组16例、50 g果糖组15例和75 g果糖组14例完成研究。25 g果糖组、50 g果糖组和75 g果糖组服糖后ALT和AST增幅明显高于75 g葡萄糖组,差异有统计学意义(P<0.05);四组服糖后LDH变化比较差异无统计学意义(P>0.05)。服糖后180 min受试者工作特征(ROC)曲线分析结果显示,四组ALT、AST和LDH曲线下面积比较差异无统计学意义(P>0.05)。四组服糖前SOD比较差异无统计学意义(P>0.05),50 g果糖组和75 g果糖组服糖后60 min SOD及25 g果糖组、50 g果糖组和75 g果糖组服糖后180 min SOD明显低于75 g葡萄糖组[(4.84±1.88)和(4.38±1.12)μg/L比(6.25±1.65)μg/L、(4.46±1.66)、(5.22±1.66)和(3.99±0.96)μg/L比(6.55±1.78)μg/L],差异有统计学意义(P<0.05);四组服糖前后JNK、GPX、MDA和8-OHdG变化比较差异无统计学意义(P>0.05)。服糖后180 min ROC曲线分析结果显示,75 g果糖组SOD曲线下面积明显小于75 g葡萄糖组(9.06±1.88比12.74±3.15),差异有统计学意义(P<0.05);四组GPX、JNK、MDA和8-OH-dG曲线下面积比较差异无统计学意义(P>0.05)。结论一次果糖摄入可导致健康青年人血液抗氧化能力下降,氧化损伤增强,以及肝酶升�Objective To investigate the short-term effects of acute fructose intake on serum antioxidant capacity and liver enzymes in healthy young adults.Methods From January to June 2019,64 healthy young subjects were recruited,and divided into 75 g glucose group,25 g fructose group,50 g fructose group and 75 g fructose group by random digits table method with 16 cases each.The subjects took corresponding amounts of glucose or fructose according to grouping.The levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),lactate dehydrogenase(LDH),glutathione peroxidase(GPX),superoxide dismutase(SOD),C-Jun amino terminal kinase(JNK),malondialdehyde(MDA)and 8-OH deoxyguanine(8-OHdG)before taking sugar and 30,60,120,180 min after taking sugar,and the changes of ALT,AST and LDH at 30,60,120 and 180 min after taking sugar compared with that before taking sugar.Results One case in 50 g fructose group,2 cases in 75 g fructose group and 1 case in 75 g glucose group dropped out due to adverse reaction;finally,15 cases in 75 g glucose group,16 cases in 25 g fructose group,15 cases in 50 g fructose group and 14 cases in 75 g fructose group completed the study.The increase of ALT and AST after taking sugar in 25 g fructose group,50 g fructose group and 75 g fructose group was significantly higher than that in 75 g glucose group,and there were statistical differences(P<0.05);there was no statistical difference in the change of LDH after taking sugar among 4 groups(P>0.05).One hundred and eighty min after taking sugar,the receiver operating characteristic(ROC)curve analysis result showed that there were no statistical differences in the areas under curve of ALT,AST and LDH among 4 groups(P>0.05).There was no statistical difference in SOD before taking sugar among 4 groups(P>0.05);the SOD 60 min after taking sugar in 50 g fructose group and 75 g fructose group,and SOD 180 min after taking sugar in 25 g fructose group,50 g fructose group and 75 g fructose group were significantly lower than those in 75 g glucose group:(4.84�

关 键 词：果糖 尿酸 丙氨酸转氨酶 转氨酶类 抗氧化酶 

分 类 号：R589.7[医药卫生—内分泌]