超声辅助绿色低共熔溶剂提取番石榴叶总黄酮的工艺研究  

Ultrasonic-assisted extraction of total flavonoids from guava leaves by natural deep eutectic solvents

作　　者：蒋利荣 何海燕[1,2] 覃拥灵 陈莉莉[1] 伍荟西 莫倩涵[1] 冯建海 张高荣 JIANG Li-rong;HE Hai-yan;QIN Yong-ling;CHEN Li-li;WU Hui-xi;MO Qian-han;FENG Jian-hai;ZHANG Gao-rong

机构地区：[1]河池学院化学与生物工程学院,广西河池546300 [2]河池学院微生物及植物资源开发利用广西高校重点实验室,广西河池546300

出　　处：《饲料研究》2022年第12期72-76,共5页Feed Research

基　　金：广西自然科学基金项目(项目编号:2020GXNSFAA297218);广西高校中青年教师基础能力提升项目(项目编号:KY2016LX281、2020KY15019、2022ky0611);大学生创新创业训练计划项目(项目编号:202210605032、S202210605164S)。

摘　　要：研究以低共熔溶剂结合超声辅助萃取技术提取番石榴叶总黄酮。以番石榴叶为原料,低共熔溶剂为溶媒,在单因素试验基础上,分析低共熔溶剂组成、氢键受体和供体的物质的量的比、液料比、提取时间对番石榴叶总黄酮提取率的影响,利用Box-Behnken原理设计响应面试验,优化提取工艺参数。结果显示:低共熔溶剂氯化胆碱-乙二醇效果较其他溶剂效果好,物质的量的比1∶15、提取时间45 min、液料比17 mL/g是最佳提取参数,得到总黄酮提取率为21.01%,相对标准偏差(RSD)为1.1%,与回归模型预测值相差0.98%。研究表明,以氯化胆碱-乙二醇体系的低共熔溶剂可用于番石榴叶总黄酮的提取,经响应面优化的工艺提取效果良好。In the study,a highly and efficient extraction technique for determination of flavonoids from guava leaves using a combination of ultrasound-assisted extraction and deep eutectic solvents(DES)was developed.Using guava leaves as raw materials and deep eutectic solvents as solvent,response surface experiments were designed based on Box-Behnken principle.The effect of deep eutectic solvents composition,the ratio of moles of hydrogen bond acceptor and donor,liquid-solid ratio and extraction time on extraction yield of total flavonoids from guava leaves were analyzed,and the extraction parameters were optimized.The results showed that deep eutectic solvents choline chloride/ethylene glycol was better than other solvents.The ratio of moles 1∶15,extraction time 45 min and liquid-solid ratio 17 mL/g were the best extraction parameters.The extraction yield of total flavonoids was 21.01%,RSD was 1.1%,0.98%different from the predicted value of regression model.The study indicates that the deep eutectic solvents of choline chloride/ethylene glycol system can be used for the extraction of total flavonoids from guava leaves,and the extraction effect is good with the optimization of forward response surface.

关 键 词：低共熔溶剂 黄酮 番石榴叶 响应面分析 氯化胆碱 超声辅助萃取 

分 类 号：S816.7[农业科学—饲料科学]