解钾细菌对滇重楼叶片面积、光合色素含量及生理生化的影响  

Effect of Potassium Solubilizing Bacteria on Leaf Area,Photosynthetic Pigment Content,Physiology and Biochemistry of Paris polyphylla var.yunnanensis

作　　者：王丹 赵顺鑫 吴洋桢 吴应梅 周浓 WANG Dan;ZHAO Shunxin;WU Yangzhen;WU Yingmei;ZHOU Nong(The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area´s Medicinal Herbs,College of Food and Biological Engineering,Chongqing Three Gorges University,Chongqing 404120,China)

机构地区：[1]重庆三峡学院生物与食品工程学院,三峡库区道地药材绿色种植与深加工重庆市工程实验室,重庆404120

出　　处：《中国实验方剂学杂志》2022年第16期150-155,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：重庆市自然科学基金项目(cstc2018jcyjAX0267);“成渝地区双城经济圈建设”科技创新项目(KJCX2020046)。

摘　　要：目的:研究接种解钾细菌对滇重楼生理特性的影响。方法:通过室温盆栽实验,研究接种不同解钾细菌对滇重楼叶片面积、光合色素含量及叶片生理指标的影响。结果:接种解钾细菌后,滇重楼叶面积有一定程度的增加,平均叶面积最大的是S3组,达到了10.497 c^(m2),较对照组最大增幅为11.0%;接种解钾细菌能提高叶片中光合色素的含量,其中,叶绿素a的含量明显增加(P<0.05),较CK组最大增幅为80.6%,且所有处理组的叶绿素a/b值均高于CK组;接种解钾细菌后滇重楼叶片中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性增强,与CK组比较最大增幅分别为88.4%、33.8%和30.6%;接种解钾细菌能促进滇重楼叶片细胞对渗透调节物质可溶性糖和可溶性蛋白的积累,较CK组最大增幅分别为55.3%、70.5%,同时还降低丙二醛(MDA)的含量,较CK组最大降幅为34.1%;相关性分析结果显示,滇重楼叶片中不同化学成分指标之间部分存在相关性。结论:解钾细菌能有效促进滇重楼的生长发育,提高滇重楼在逆境中的生存能力,对于提高滇重楼人工种植存活率具有一定的科学意义和潜在的应用价值。Objective:To investigate the effects of inoculation of potassium-solubilizing bacteria on the physiological characteristics of Paris polyphylla var.yunnanensis.Method:The effects of different potassiumsolubilizing bacteria on leaf area,photosynthetic pigment content,and active component indexes of P.polyphylla var.yunnanensis were investigated by pot planting at room temperature.Result:The results showed that the inoculation of potassium-solubilizing bacteria could increase the leaf area of P.polyphylla var.yunnanensis.The largest average leaf area was observed in the S3 group,reaching 10.497 c^(m2),with a maximum increase of 11.0%compared with that of the CK group.The inoculation of potassium-solubilizing bacteria could increase the content of photosynthetic pigments in leaves.Among them,the increase in chlorophyll a content was significant(P<0.05),which was 80.6%higher than that of the CK group.The chlorophyll a/b values in all treatment groups were higher than that of the CK group.The activities of superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD)in the leaves of P.polyphylla var.yunnanensis increased by 88.4%,33.8%,and 30.6%,respectively,as compared with that in the CK group.The inoculation of potassium-solubilizing bacteria could promote the accumulation of osmoregulation substances such as soluble sugar and soluble protein in leaf cells of P.polyphylla var.yunnanensis,with a maximum increase of 55.3%and 70.5%respectively compared with that of the CK group.Meanwhile,it also reduced the content of malondialdehyde(MDA),with a maximum decrease of 34.1%compared with that of the CK group.The results of correlation analysis showed that there were some correlations between different chemical components in P.polyphylla var.yunnanensis leaves.Conclusion:The inoculation of potassium-solubilizing bacteria can effectively promote the growth and development of P.polyphylla var.yunnanensis and improve its survival ability in adversity.It is of practical scientific significance and potential application value for

关 键 词：滇重楼 解钾细菌 生理生化 活性成分 

分 类 号：R284.2[医药卫生—中药学] R289[医药卫生—中医学] R22R2-031R33