Exploring key developmental phases and phase-specific genes across the entirety of anther development in maize  

作　　者：Yingjia Han Mingjian Hu Xuxu Ma Ge Yan Chunyu Wang Siqi Jiang Jinsheng Lai Mei Zhang 

机构地区：[1]Key Laboratory of Plant Molecular Physiology,Institute of Botany,The Chinese Academy of Sciences,Beijing 100093,China [2]State Key Laboratory of Plant Physiology and Biochemistry&National Maize Improvement Center of China Department of Plant Genetics and Breeding,China Agricultural University,Beijing 100193,China [3]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Integrative Plant Biology》2022年第7期1394-1410,共17页植物学报（英文版）

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA24010106);National Natural Science Foundation of China(32072075);National Center for Biotechnology Information’s Short Read Archive under BioProject accession PRJNA750514。

摘　　要：Anther development from stamen primordium to pollen dispersal is complex and essential to sexual reproduction.How this highly dynamic and complex developmental process is controlled genetically is not well understood,especially for genes involved in specific key developmental phases.Here we generated RNA sequencing libraries spanning 10 key stages across the entirety of anther development in maize(Zea mays).Global transcriptome analyses revealed distinct phases of cell division and expansion,meiosis,pollen maturation,and mature pollen,for which we detected 50,245,42,and 414 phase-specific marker genes,respectively.Phase-specific transcription factor genes were significantly enriched in the phase of meiosis.The phase-specific expression of these marker genes was highly conserved among the maize lines Chang7-2 and W23,indicating they might have important roles in anther development.We explored a desiccationrelated protein gene,ZmDRP1,which was exclusively expressed in the tapetum from the tetrad to the uninucleate microspore stage,by generating knockout mutants.Notably,mutants in ZmDRP1 were completely male-sterile,with abnormal Ubisch bodies and defective pollen exine.Our work provides a glimpse into the gene expression dynamics and a valuable resource for exploring the roles of key phase-specific genes that regulate anther development.

关 键 词：anther development MAIZE MALE-STERILE phase-specific genes TRANSCRIPTOME ZmDRP1 

分 类 号：S513[农业科学—作物学]