毛细管多重电泳技术鉴定水稻种子纯度研究  

Preliminary Study on the Identification of Rice Seed Purity by Multiple Capillary Electrophoresis

作　　者：范家萌 王至严 林慧慧 周桂林[1] 王浩波[1] FAN Jia-meng;WANG Zhi-yan;LIN Hui-hui(Hefei Fengle Seed Industry Co.,Ltd.,Key Laboratory of Anhui Province for New Techniques and Varieties of Crop Seeds,Hefei,Anhui 230088)

机构地区：[1]合肥丰乐种业股份有限公司,农作物种子新技术与新品种创制安徽省重点实验室,安徽合肥230088

出　　处：《安徽农业科学》2022年第15期90-94,共5页Journal of Anhui Agricultural Sciences

基　　金：合肥市关键技术“借转补”研发项目(J2020G44);农作物种子新技术与新品种创制安徽省重点实验室(2017010205)。

摘　　要：[目的]建立一种混合多个PCR产物的高效单色毛细管电泳技术。[方法]以杂交水稻丰两优3305和常规稻润稻118为试验材料,结合品种指纹数据和PCR产物片段大小之间的差异,筛选出适用于该品种纯度鉴定的SSR分子标记。通过单重和多重毛细管电泳结果比对,验证多重电泳技术应用于SSR分子标记检测水稻纯度的实用性。[结果]片段差异10 bp及以上不同分子标记的PCR产物可以进行多重电泳。多重毛细管电泳技术既能降低检测成本,又能提高试验效率,可进一步提升毛细管电泳仪使用效能。[结论]该研究可为高效检测水稻种子纯度提供技术参考。[Objective]To establish a high-efficiency single-color capillary electrophoresis technology for mixing multiple PCR products.[Method]The hybrid rice Fengliangyou 3305 and the conventional rice Rundao 118 were used as experimental materials,based on the difference between cultivar fingerprint data and PCR product fragment size,to screen the SSR molecular markers suitable for the purity identification of this variety.Comparison of singleplex and multiplex capillary electrophoresis results,to verify the practicability of multiplex electrophoresis technology applied to SSR molecular markers to detect the purity of rice.[Result]The results showed that PCR products with different molecular markers with a fragment difference of 10 bp or more can be multiplex electrophoresed.Multiple capillary electrophoresis technology can not only reduce the detection cost but also improve the experimental efficiency,it can further improve the performance of the capillary electrophoresis instrument.[Conclusion]The study can provide a technical reference for the efficient detection of rice seed purity.

关 键 词：水稻 SSR分子标记 纯度鉴定 多重电泳 

分 类 号：S511[农业科学—作物学]