灵芝UDP-GlcDH基因的cDNA克隆及生物信息学分析  

cDNA Cloning and Bioinformatics Analysis of UDP-GlcDH Gene from Ganoderma lucidum

作　　者：章鸿宇 张燎原[1] ZHANG Hong-yu;ZHANG Liao-yuan(College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学生命科学学院,福建福州350002

出　　处：《福建农业科技》2022年第5期12-17,共6页Fujian Agricultural Science and Technology

基　　金：福建省科技计划星火项目(KJF20H05A)。

摘　　要：尿苷二磷酸葡萄糖脱氢酶(UDP glucose dehydrogenase,UDP-GlcDH)可将UDP葡萄糖催化生成多糖形成必需的前体物质UDP葡萄糖醛酸。以黄曲霉的UDP-GlcDH基因序列为模板,采用电子克隆的方法,搜索灵芝EST文库,拼接获得灵芝UDP-GlcDH基因的cDNA序列,并通过RT-PCR试验进行验证。采用生物信息学方法对UDP-GlcDH基因进行分析,表明该cDNA序列长度为1 591bp,其中包含能编码471个氨基酸的序列,分子量为51.8kD,PI为6.29,存在于内质网膜中,与污叉丝孔菌和云芝等真菌中的UDP-GlcDH基因编码的氨基酸序列高度同源。UDP glucose dehydrogenase(UDP-GlcDH)can catalyze UDP glucose to UDP glucuronic acid,which is an essential precursor for the formation of polysaccharide.The UDP-GlcDH gene sequence of Aspergillus flavus was used as the template,and the cDNA sequence of UDP-GlcDH gene from Ganoderma lucidum was obtained by searching the EST library of Ganoderma lucidum through the electronic cloning method,and then verified by using the RT-PCR testing.The bioinformatics method was used to analyze the UDP-GlcDH gene,showing that the cDNA sequence was 1591 bp in length,which contained 471 amino acid sequences with the molecular weight of 51.8 kD and PI of 6.29.The UDP-GlcDH gene was found in the endoplasmic reticulum,which was highly homologous to the amino acid sequence encoded by UDP-GlcDH gene in the fungi such as Dichomitus squalens and Coriolus versicolor.

关 键 词：灵芝 尿苷二磷酸葡萄糖脱氢酶 电子克隆 生物信息学分析 

分 类 号：S567.31[农业科学—中草药栽培]