Sirt1对小鼠肝再生影响的研究  

Studies of Sirt1 in Liver Regeneration

作　　者：魏韬[1] 周晶琳[1] 徐绍娜 刘洋[1] 陆迪[1] 祝慧慧[1] WEI Tao;ZHOU Jing-lin;XU Shao-na;LIU Yang;LU Di;ZHU Hui-hui(Basic Medical School,Mudanjiang Medical University,Mudanjiang Heilongjiang,157011,China)

机构地区：[1]牡丹江医学院基础医学院,黑龙江牡丹江157011

出　　处：《中华养生保健》2022年第16期1-4,共4页CHINESE HEALTH CARE

基　　金：黑龙江省省属高等学校基本科研业务费生物医药类支持计划项目(2018-KYYWFMY-0028);黑龙江省卫生健康委科研课题(2018504)。

摘　　要：目的调查肝切除术后,小鼠肝再生过程中组蛋白去乙酰基转移酶Sirt1转录水平的变化,研究Sirt1在肝再生中可能的作用。方法以70%肝切除小鼠为模型,分别于术后12 h、24 h、48 h和72 h,从实验组中随机选取3只小鼠并处死,对照组在假手术后立即处死。通过肝脏体质量比评估小鼠肝再生能力;使用血清谷丙转氨酶(ALT)测定肝损伤程度;采用荧光定量PCR(RT-qPCR)法检测小鼠肝组织中Sirt1的转录水平。结果肝切除术后小鼠肝脏体质量比逐渐增加;术后12 h、24 h和48 h血清ALT水平均高于对照组,术后72 h基本恢复;术后48 h Sirt1转录水平降低。结论小鼠行肝切除术后,能够迅速启动肝再生过程,其机制可能与Sirt1转录水平降低,进而导致某些蛋白乙酰化水平升高有关。Objective To investigate the role and transcriptional level of histone deacetyltransferase Sirt1 in liver regeneration after hepatectomy.Methods Three mice were randomly selected and executed at 12 h,24 h,48 h and 72 h postoperatively in the experimental group,and the mice in control group were executed immediately after the sham operation.The ability of liver regeneration was assessd by liver weight/body weight ratio,the degree of liver injury was detected by ALT activity detection and the transcriptional level of histone deacetyltransferase Sirt1 in mouse liver tissue was detected by real-time quantitative PCR(RT-qPCR).Results Aftter hepatectomy,the liver weight/body weight ratio in the experimental group increased gradually.compared with the control group,ALT levels in serum were higher at 12 h,24 h and 48 h,and recovered at 72 h.At 48 h,the transcriptional level of histone deacetyltransferase Sirt1 decreased.Conclusion Liver regeneration after hepatectomy in mice might be related to the decrease of histone deacetyltransferase Sirt1 transcription,which resulted in the increase of acetylation of some proteins.

关 键 词：肝切除 乙酰化 肝再生 

分 类 号：R3[医药卫生—基础医学]