风流草黄酮对大鼠骨折愈合的作用研究  

Effect of Desmodium gyrans(L.)DC.Flavonoids on Fracture Healing in Rats

作　　者：孙雅嘉 孙九许 燕梦云 丁凌 裴凌鹏 SUN Yajia;SUN Jiuxu;YAN Mengyun;DING Ling;PEI Lingpeng(School of Pharmacy,Minzu University of China,Beijing 100081,China;Key Laboratory of Ethnomedicine,Ministry of Education,Beijing 100081,China)

机构地区：[1]中央民族大学药学院,北京100081 [2]民族医药教育部重点实验室,北京100081

出　　处：《中国中医基础医学杂志》2022年第7期1081-1084,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(81473451)-基于3D打印技术与分子信号网络理论研究刺老苞根皮有效成分群调控骨折愈合作用机制;国家自然科学基金资助项目(81673769)-基于信号网络耦联节点理论探讨刺老苞根皮有效成分群干预糖尿病骨质疏松症的分子机制研究。

摘　　要：目的:探究风流草黄酮对胫骨骨折模型大鼠骨折愈合过程中骨质变化的影响。方法:将300只6周龄SD大鼠按照给药周期(4,7,14,21,28 d)随机分为5大组,每大组60只。再将每大组按处理方式分为对照组、模型组、风流草黄酮低剂量组(15 mg/kg)、中剂量组(30 mg/kg)以及高剂量组(60 mg/kg)各12只,分别于给药4、7、14、21、28 d后用Elisa法测定相应组别大鼠胫骨中碱性磷酸酶(alkaline phosphatase,ALP)活性、总胶原蛋白及羟脯氨酸(hydroxyproline,Hyp)含量,放射免疫法测定骨钙素(osteocalcin,OCN)含量,电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry,ICP-MS)测定骨钙、骨磷含量,并比较组间差异。结果:与对照组比较,模型组不同时间点的骨代谢相关参数均有所降低(P<0.01)。与模型组比较,风流草黄酮不同剂量组不同时间点的骨代谢相关参数均有所升高(P<0.05,P<0.01),且呈时间、剂量依赖性。结论:风流草黄酮可改善胫骨骨折大鼠的骨代谢状况,促进骨形成,有助于骨折愈合。Objective:To explore the effect of Desmodium gyrans(L.)DC.flavonoids on bone changes during fracture healing in tibial fracture model rats.Methods:According to different administration time(4,7,14,21,28 days),a total of 3006-week-old SD rats were randomly divided into five large groups,with 60 rats in each large group.Then,according to different treatment methods,each large group was divided into control group,model group,and low dose,medium dose,high dose(15,30,60mg/kg)of Desmodium gyrans(L.)DC.flavonoids group,with 12 rats in each group.On the 4^(th) day,7^(th) day,14^(th) day,21^(st) day and 28^(th) day,tibia osseous routine parameters of corresponding groups of rats were tested and compared respectively.Alkaline phosphatase(ALP)activity,total bone collagen and hydroxyproline(Hyp)contents were determined by Elisa.The content of osteocalcin(OCN)was determined by radioimmunoassay.Bone calcium and phosphorus contents were determined by inductively coupled plasma mass spectrometry(ICP-MS).Results:Compared with control group,osseous routine parameters in the model group were significantly decreased(P<0.01).However,osseous routine parameters changes in the Desmodium gyrans(L.)DC.flavonoids groups significantly increased and presented time and dose dependence,compared with the model group(P<0.05,P<0.01).Conclusion:Desmodium gyrans(L.)DC.flavonoids can play a positive role in bone metabolism and osteogenesis of tibia fracture model in SD rat,which suggest it can be benefit for fracture healing.

关 键 词：风流草 黄酮 骨折愈合 骨质代谢 

分 类 号：R285.5[医药卫生—中药学]