吗啡成瘾消退及纳洛酮对前额叶脑电功率谱的影响  

Effects of extinction of morphine addiction and naloxone on the EEG power spectrum in the prefrontal cortex

作　　者：李春春 王晓琴[2] 王功伍 曹军[3] LI Chunchun;WANG Xiaoqin;WANG Gongwu;CAO Jun(MOE Engineering Research Center of Sustainable Development and Utilization of Bioenergy,Kunming&Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment,School of Life Sciences,Kunming 650500,China;School of Physical Education,Yunnan Normal University,Kunming 650500,China;Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology,School of Ecology and Environmental Science,Yunnan University,Kunming 650500,China;NHC Key Laboratory of Drug Addiction Medicine,The First Affiliated Hospital of Kunming Medical University,Kunming 650032,China)

机构地区：[1]云南师范大学生命科学学院生物能源持续开发利用教育部工程研究中心和云南省生物质能与环境生物技术重点实验室,昆明650500 [2]云南师范大学体育学院,昆明650500 [3]云南大学生态与环境学院云南省植物繁殖适应与进化生态学重点实验室,昆明650500 [4]昆明医科大学第一附属医院国家卫健委毒品依赖和戒治重点实验室,昆明650032

出　　处：《生物学杂志》2022年第4期12-17,共6页Journal of Biology

基　　金：国家自然科学基金项目(31760278);国家卫生健康委员会毒品依赖和戒治重点实验室项目(2020DAMOP-002)。

摘　　要：为探讨训练消退(TE)和自然消退(NE)对吗啡(MOP)成瘾小鼠的前额叶(PFC)脑电(EEG)功率谱的影响及纳洛酮(NLX)的调控作用,将昆明小鼠以剂量递增方式给药(MOP:10、15、20、25和30 mg/kg)或生理盐水(SAL),训练10 d,建立条件位置偏爱(CPP)模型。消退5 d后,采集PFC-EEG,并注射NLX(5 mg/kg)。结果发现:小鼠MOP-CPP模型建成后,经5 d消退,MOP-TE组CPP分数降至基线和SAL组水平,但其EEG总功率下降(P<0.05);MOP-NE组CPP分数仍然较高,其总功率却与SAL组相似(P>0.05)。NLX注射后,MOP-TE组功率提高较快,其θ、α、β、γ_(1)频段功率也有类似表现。结果提示,PFC-EEG功率谱是PFC参与阿片类药物成瘾消退的重要电生理指标,而NLX在其中有一定改善作用。To investigate the effects of training extinction(TE)and natural extinction(NE)on the EEG power spectrum of the prefrontal cortex(PFC)of mice with morphine(MOP)addiction and the regulation of naloxone(NLX),KM mice were given increasing doses of MOP(10,15,20,25,30 mg/kg)or saline(SAL)for 10 d,and the conditioned position preference(CPP)model was established.After 5 d extinction,the PFC-EEG signal was recorded with NLX(5 mg/kg)treatment.Results showed that,after 5 d TE treatment,the CPP score in the MOP-TE group decreased to the level as its baseline or the SAL group,with the total EEG power decreased(P<0.05).The CPP score was still higher in MOP-NE group,with the total power similar to that in SAL group(P>0.05).After NLX injection,the power of MOP-TE group increased rapidly,and the power ofθ,α,βandγ1 bands also showed similar performance.The results suggested that the PFC-EEG power spectrum would be an important electrophysiological index on involvement of the PFC in extinction of opioid drug addiction,and in which,the NLX would have a certain improvement effect.

关 键 词：吗啡成瘾 纳洛酮 条件位置偏爱 前额叶 脑电功率谱 昆明小鼠 

分 类 号：Q189[生物学—神经生物学] Q426[生物学—普通生物学]