一株藻际芽孢杆菌对条斑紫菜生长及生理的影响  

Effects of a strain of Bacillus spp.on the growth and physiology of Pyropia yezoensis

作　　者：欧阳乐飞 王林华 段健诚 张庆起 高焕 阎斌伦 OUYANG Lefei;WANG Linhua;DUAN Jiancheng;ZHANG Qingqi;GAO Huan;YAN Binlun(Jiangsu Key Laboratory of Marine Biotechnology,Key Laboratory of Marine Biological Resources and Ecological Environment of Jiangsu Province,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Marine Biological Industry Technology Collaborative Innovation Center,Lianyungang 222005,China;Ganyu-jiaxin Aquatulure Limited Company of Lianyungang,Lianyungang 222100,China;Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Nanjing 210014,China)

机构地区：[1]江苏海洋大学江苏省海洋生物技术重点实验室江苏省海洋生物资源与生态环境重点实验室,连云港222005 [2]江苏省海洋生物产业技术协同创新中心,连云港222005 [3]连云港赣榆佳信水产开发有限公司,连云港222100 [4]江苏省农业种质资源保护与利用平台,南京210014

出　　处：《生物学杂志》2022年第4期44-49,共6页Journal of Biology

基　　金：国家现代农业产业体系-藻类体系项目(CARS-50);江苏省水产三新工程项目(Y2017-32);江苏海洋大学研究生创新项目(SJCX19-0995);江苏省优势学科建设工程资助项目。

摘　　要：为阐释藻际芽孢杆菌对条斑紫菜生长生理的影响,以条斑紫菜(Pyropia yezoensis)叶状体及其一种附生菌——芽孢杆菌(Bacillus sp.LPyS1)为材料,研究不同时间点(0、6、12、24、48、72 h)藻-菌共培养后芽孢杆菌对条斑紫菜叶状体相对生长率(RGR)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、光和色素(Chla)、藻胆蛋白(RPE、RPC、APC)、总蛋白(TP)、丙二醛(MDA)和游离脯氨酸(Pro)含量的影响。结果表明:与对照组相比,短期内芽孢杆菌可显著促进条斑紫菜RGR上升(P<0.05),但对条斑紫菜叶状体SOD、POD的活性和丙二醛(MDA)含量无显著性影响(P>0.05);培养24 h后,共培养组与对照组条斑紫菜均产生过量渗透调节物质,其中总蛋白、光合色素、藻胆蛋白共培养组在48 h均显著高于对照组(P<0.05),而Pro含量在48 h极显著低于对照组(P<0.01)。研究表明,芽孢杆菌(Bacillus sp.LPyS1)有利于促进条斑紫菜生长,推测该藻际附生菌可能为藻际环境中的条斑紫菜提供营养物质,从而影响条斑紫菜的生长与生理状态。To explain the effect of Bacillus spp.on the growth and physiology of Pyropia yezoensis,Pyropia yezoensis fronds and one of its epiphytic bacteria-Bacillus sp.LPyS1 were used as materials.Effects of the different time points(0,6,12,24,48,72 h),the relative growth rate(RGR),the activity of superoxide dismutase(SOD),peroxidase(POD),phycobiliprotein(Chla),photosynthetic pigments(RPE,RPC and APC),total protein(TP),malondialdehyde(MDA)and free proline(Pro)content were studied.The results showed that,compared with the control group,Bacillus could significantly increase the RGR of Pyropia yezoensis in a short period of time(P<0.05),Bacillus had no significant effect on the activity of SOD,POD and the content of malondialdehyde(MDA)in Pyropia yezoensis fronds(P>0.05).After 24 h of culture,the co-culture group and control group Pyropia yezoensis produced excessive osmotic adjustment substances.The total protein,photosynthetic pigment and phycobiliprotein of co-culture group were significantly higher than that of the control group at 48 h(P<0.05),but the content of Pro in the co-culture group was significantly lower than that in the control group at 48 h(P<0.01).This experimental study showed Bacillus sp.LPyS1 was beneficial to promote the growth of Pyropia yezoensis.Therefore,it is speculated that this algal epiphytic bacteria might provide nutrients for Pyropia yezoensis in the algal environment,thereby affecting the growth and physiological state of Pyropia yezoensis.

关 键 词：条斑紫菜 芽孢杆菌 藻际微环境 生长 生理 

分 类 号：S968.4[农业科学—水产养殖]