TaNRT2.1-6B is a dual-affinity nitrate transporter contributing to nitrogen uptake in bread wheat under both nitrogen deficiency and sufficiency  

作　　者：Mengjiao Li Tian Wang Hui Zhang Shuo Liu Wenhu Li Salah F.Abou Elwafa Hui Tian 

机构地区：[1]Key Laboratory of Plant Nutrition and Agri-environment in Northwest China,Ministry of Agriculture and Rural Affairs,College of Natural Resources and Environment,Northwest A&F University,Yangling 712100,Shaanxi,China [2]Agronomy Department,Faculty of Agriculture,Assiut University,Assiut 71526,Egypt

出　　处：《The Crop Journal》2022年第4期993-1005,共13页作物学报（英文版）

基　　金：funded by the National Natural Science Foundation of China(31972497).

摘　　要：Multiple nitrate transporter(NRT)genes exist in the genome of bread wheat,and it is of great importance to identify the elite NRT genes for N-efficient wheat cultivar breeding.A candidate gene association study(CGAS)of six N use efficiency(NUE)related traits(grain N concentration(GNC),straw N concentration(SNC),grain yield(GY),grain N accumulation(GNA),shoot total N accumulation(STN)and N harvest index(NHI))was performed based on SNPs in 46 NRT2 genes using a panel composed of 286 wheat cultivars.CGAS identified TaNRT2.1-6B as an elite NRT gene that is significantly associated with four(NHI,SNC,GNA and GY)of the six NUE-related traits simultaneously.TaNRT2.1-6B is located on the plasma membrane and acts as a dual-affinity NRT.The overexpression of TaNRT2.1-6B increased the N influx and root growth of wheat,whereas gene silence lines resulted in the opposite effects.The overexpression of TaNRT2.1-6B also improved GY and N accumulation of wheat under either limited or sufficient N conditions.The data provide the TaNRT2.1-6B gene and the two associated SNP markers as promising powerful tools for breeding wheat cultivars with high N uptake ability and NUE.

关 键 词：Candidate gene association study WHEAT TaNRT2.1-6B Dual-affinity transporter Nitrogen use efficiency 

分 类 号：S512.1[农业科学—作物学]