miR398b negatively regulates cotton immune responses to Verticillium dahliae via multiple targets  

作　　者：Yuhuan Miao Kun Chen Jinwu Deng Lin Zhang Weiran Wang Jie Kong Steven J.Klosterman Xianlong Zhang Alifu Aierxi Longfu Zhu 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,Hubei,China [2]Hubei University of Chinese Medicine,Wuhan 430065,Hubei,China [3]Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,Hubei,China [4]Institute of Economic Crops,Xinjiang Academy of Agricultural Sciences,Urumchi 842000,Xinjiang,China [5]United States Department of Agriculture,Agricultural Research Service,Salinas,CA 93905,USA

出　　处：《The Crop Journal》2022年第4期1026-1036,共11页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2018YFD0100403);the project from the Ministry of Science and Technology of China(KY201702009).

摘　　要：MicroRNAs(miRNAs)play essential roles in plant defense responses,although such roles have not been identified in cotton in response to the plant pathogenic fungus Verticillium dahliae.In this study,the functions of miR398b and its target genes in cotton-V.dahliae interaction were investigated.The transcript levels of miR398b were down-regulated by V.dahliae infection and miR398b overexpression in cotton made the plants more susceptible to V.dahliae.The results suggest that miR398b negatively regulates cotton resistance to V.dahliae.This may occur by miR398b repression of some CC-NBS-LRR genes via translational inhibition,interfering with defense responses and leading to cotton susceptibility to V.dahliae.Alternatively,miR398b may guide the cleavage of the mRNAs of GhCSD1,GhCSD2 and GhCCS,each of which functions in reactive oxygen species(ROS)regulation and homeostasis,thereby causing excessive ROS accumulation in miR398b-overexpressing plants in response to V.dahliae infection.This study suggests conserved and novel roles of miR398b in the cotton–V.dahliae interaction.These discoveries may be coupled with new strategies in cotton breeding programs to improve resistance to V.dahliae.

关 键 词：COTTON miR398b NBS-LRR ROS homeostasis Verticillium dahliae 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]