Genetic analysis and gene mapping of a dwarf and liguleless mutation in barley  

作　　者：Baojian Guo Jiang Qi Dongfang Li Hongwei Sun Chao Lyu Feifei Wang Juan Zhu Ganggang Guo Rugen Xu 

机构地区：[1]Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology,Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009,Jiangsu,China [2]Joint International Research Laboratory of Agriculture and Agri-Product Safety,The Ministry of Education of China,Yangzhou University,Yangzhou 225009,Jiangsu,China [3]Key Laboratory of Crop Germplasm Resources and Utilization(MOA),The National Key Facility for Crop Gene Resources and Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《The Crop Journal》2022年第4期1094-1102,共9页作物学报（英文版）

基　　金：supported by the Open Project Program of Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University (JILARKF202002);Natural Science Foundation of the Jiangsu Higher Education Institutions of China (19KJA560005);China Agriculture Research System of MOF and MARA (CARS-05);a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions

摘　　要：Leaf development underlies crop growth and productivity and has been a major target of crop domestication and improvement.However,most genes controlling leaf development in barley remain unknown.We identified a dwarf and liguleless(dl)mutant derived by ethylmethane sulfonate mutagenesis.The dl mutant showed dramatic changes in shoot architecture compared with wild-type(Yangnongpi 5)plants.Besides lacking ligules,the dl mutant showed much shorter plant height(28 cm)than Yangnongpi 5(78 cm).By map-based cloning,the dl gene was localized to a 56.58-kb genomic interval on the long arm of chromosome 7.A C-to-T single-nucleotide substitution was identified at exon position 790,and is a functional mutation resulting in a proline-to-serine substitution at the 264th amino acid residue of HORVU7Hr1G106960.Consequently,HORVU7Hr1G106960 was identified as the DL gene,encoding 269 amino acids and containing the Arabidopsis LSH1 and Oryza G1(ALOG)domain.DL is highly similar to rice OsG1-LIKE 1/2(OsG1L1/2)and sorghum AWN1/AWN1-10 at the amino acid level.Although the dl mutant allele showed no expression changes in selected tissues by real-time PCR,we propose HORVU7Hr1G106960 as a candidate gene conferring the dwarf and liguleless phenotype in barley.

关 键 词：BARLEY EMS Liguleless ALOG Shoot architecture 

分 类 号：S512.3[农业科学—作物学]