Fine mapping of an adult-plant resistance gene to powdery mildew in soybean cultivar Zhonghuang 24  

作　　者：Qian Zhou Bingzhi Jiang Yanbo Cheng Qibin Ma Qiuju Xia Ze Jiang Zhandong Cai Hai Nian 

机构地区：[1]The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,South China Agricultural University,Guangzhou 510642,Guangdong,China [2]The Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology,South China Agricultural University,Guangzhou 510642,Guangdong,China [3]The Key Laboratory of Plant Molecular Breeding of Guangdong Province,College of Agriculture,South China Agricultural University,Guangzhou 510642,Guangdong,China [4]Guangdong Provincial Key Laboratory of Crops Genetics and Improvement,Crops Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510642,Guangdong,China [5]Beijing Genomics Institute(BGI)Education Center,University of Chinese Academy of Sciences,Shenzhen 518083,Guangdong,China

出　　处：《The Crop Journal》2022年第4期1103-1110,共8页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31971966);the Key-Areas Research and Development Program of Guangdong Province(2020B020220008);the China Agriculture Research System(CARS-04-PS09).

摘　　要：Powdery mildew(PM),caused by the fungus Microsphaera diffusa,causes severe yield losses in soybean[Glycine max(L.)Merr.]under suitable environmental conditions.Identifying resistance genes and developing resistant cultivars may prevent soybean PM damage.In this study,analysis of F_(1),F_(2),and F8:11 recombinant inbred line(RIL)populations derived from the cross between Zhonghuang 24(ZH24)and Huaxia 3(HX3)indicated that adult-plant resistance(APR)to powdery mildew in the soybean cultivar(cv.)ZH24 was controlled by a single dominant locus.A high-density genetic linkage map of the RIL population was used for fine mapping.The APR locus in ZH24 was mapped to a 281-kb genomic region on chromosome 16.Using 283 susceptible plants of another F2 population,the candidate region was finemapped to a 32.8-kb genomic interval flanked by the markers InDel14 and Gm16_428.The interval harbored five genes,including four disease resistance(R)-like genes,according to the Williams 82.a2.v1 reference genome.Quantitative real-time PCR assays of candidate genes revealed that the expression levels of Glyma.16g214300 and Glyma.16g214500 were changed by M.diffusa infection and might be involved in disease defense.Rmd_B13 showed all-stage resistance(ASR)to PM in soybean cv.B13.An allelism test in the F2 segregating population from the cross of ZH24 × B13 suggested that the APR locus Rmd_ZH24 and the ASR locus Rmd_B13 may be allelic or tightly linked.These results provide a reference marker-assisted selection in breeding programs.

关 键 词：Glycine max Powdery mildew Adult-plant resistance Gene Fine mapping 

分 类 号：S565.1[农业科学—作物学]