中药川芎的热性物质基础探索  

Exploration on the Hot Properties Material Basis of Traditional Chinese Medicine Chuanxiong

作　　者：程薇薇 刘建利[2] 史敏[1] 毕芳芳 张露 何仕龙 高欣怡 Cheng Weiwei;Liu Jianli;Shi Min;Bi Fangfang;Zhang Lu;He Shilong;Gao Xinyi(School of Medicine,Xi'an Peihua University,Xi'an 710125,China;College of Life Science,Northwest University,Xi'an 710069,China)

机构地区：[1]西安培华学院医学院,陕西西安710125 [2]西北大学生命科学学院,陕西西安710069

出　　处：《云南化工》2022年第8期90-91,125,共3页Yunnan Chemical Technology

基　　金：西安培华学院2021年校级科研项目,《基于细胞中药学理论从蛋白表达角度探索中药寒热药性的作用靶点》PHKT2123;陕西省教育厅2020年度突发公共卫生安全专项科学研究计划,《黄芩素对小鼠急性肺炎的干预效果及其药理调节机制研究》20JG023;西安培华学院2021年大学生创新创业训练计划项目,《双歧杆菌联合左卡尼汀对菌群失调腹泻模型大鼠肠道菌群的影响》PHDC2021065。

摘　　要：采用噻唑蓝(MTT)比色法考察了4种甲基吡嗪类化合物2,5-二甲基吡嗪、2,3-二甲基吡嗪、2,3,5-三甲基吡嗪、2,3,5,6-四甲基吡嗪对离体培养的MCF-7细胞生长增殖的影响,以确定他们的寒热属性。结果表明:4种甲基吡嗪类化合物在一定浓度范围内对MCF-7生长增殖均具有促进作用。4种甲基吡嗪类化合物结构类似,寒热属性均为热性,存在一定的构-性关系;川芎嗪(2,3,5,6-四甲基吡嗪)是中药川芎的热性物质基础之一。To investigate the cold-heat properties of methylpyrazine compounds,and to explore the thermal material basis of the warming chinese medicine Chuanxiong.Four methylpyrazine compounds,2,5-dimethylpyrazine,2,3-dimethylpyrazine and 2,3,5-trimethylpyrazine were investigated by thiazolyl blue(MTT)colorimetric method,2,3,5,6-tetramethylpyrazine on the growth and proliferation of MCF-7 cells cultured in vitro to determine their cold and heat properties.The four methylpyrazine compounds could promote the growth and proliferation of MCF-7 in a certain concentration range.The structures of the four methylpyrazine compounds are similar,the cold and heat properties are all thermal,and there is a certain structure-property relationship;Ligustrazine(2,3,5,6-tetramethylpyrazine)is the thermal property of traditional chinese medicine Chuanxiong one of the material foundations.

关 键 词：川芎 热性物质基础 甲基吡嗪类化合物 MTT法 MCF-7细胞 

分 类 号：R285.1[医药卫生—中药学]