针刺对血管性认知障碍大鼠学习记忆能力和海马Ngb表达的影响  

Effect of acupuncture on learning and memory ability and hippocampal Ngb expression in rats with vascular cognitive impairment

作　　者：胡培佳[1,2] 程红亮 杨涛 柳星 李清海 张寒玉 Hu Peijia;Cheng Hongliang;Yang Tao;Liu Xing;Li Qinghai;Zhang Hanyu(Department of Endocrine,the Second Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230061,China;Acupuncture and Moxibustion Institute of Anhui Academy of Chinese Medicine,Hefei 230061,China;Department of Acupuncture,Shucheng County Hospital of Traditional Chinese Medicine,Shucheng 231300,China;Graduate School of Anhui University of Chinese Medicine,Hefei 230061,China)

机构地区：[1]安徽中医药大学第二附属医院内分泌科,合肥230061 [2]安徽省中医药科学院针灸临床研究所,合肥230061 [3]舒城县中医院针推科,舒城231300 [4]安徽中医药大学研究生院,合肥230061

出　　处：《国际中医中药杂志》2022年第6期645-649,共5页International Journal of Traditional Chinese Medicine

基　　金：安徽省自然科学基金项目(1908085QH370);安徽省高校自然科学基金重点项目(KJ2020A0428)。

摘　　要：目的探讨针刺对血管性认知障碍(VCI)模型大鼠学习记忆能力及海马区脑红蛋白(neuroglobin,Ngb)表达的影响。方法采用双侧颈总动脉反复结扎以引起缺血再灌注损伤法制备大鼠VCI模型。将造模成功大鼠按随机数字表法分为针刺组和模型组,每组12只,选取12只大鼠作为正常组。针刺组大鼠针刺"百会""风府""涌泉",10次为1个疗程,共3个疗程,疗程间停止针刺2 d。干预结束后第3天进行神经行为学评分,采用Morris水迷宫法检测各组大鼠学习记忆能力,采用Western blot法检测大鼠海马组织Ngb表达。结果与模型组比较,针刺组干预后行为学评分降低(P<0.05),逃避潜伏期明显缩短(P<0.05),穿越平台次数增多(P<0.05),海马Ngb[(1.18±0.06)比(0.98±0.04)]表达升高(P<0.05)。结论针刺"百会""风府""涌泉"可促进VCI模型大鼠海马区Ngb表达,改善其学习和记忆能力。Objective To discuss the effect of acupuncture on learning and memory ability and the expression of neuroglobin(Ngb)of the hippocampus of vascular cognitive impaired(VCI)rats.Methods VCI rats model was established by repeated ligation of the carotid arteries of the two sides to induce ischemia-reperfusion injury.The successful modeled rats were divided into acupuncture group and a model group according to the random number table method,with 12 rats in each group,and 12 rats were selected as the normal group.The rats in the acupuncture group received acupuncture at"Baihui","Fengfu"and"Yongquan",10 times as a course of treatment,a total of 3 courses,and stop acupuncture treatment for 2 days during the courses of treatment.Neurobehavioral scores were measured on the 3rd day after the intervention.Morris water maze was conducted to detect the learning and memory ability of the rats in each group,and Western blot was used to detect the expression of Ngb in the hippocampus of the rats.Results Compared with the model group,the behavioral score of the acupuncture group decreased after intervention(P<0.05),the escape latency was significantly shortened(P<0.05),the number of crossing platforms was increased(P<0.05),and the hippocampal Ngb(1.18±0.06 vs.0.98±0.04)was significantly increased(P<0.05).Conclusion Acupuncture at"Baihui","Fengfu"and"Yongquan"could promote the expression of Ngb in the hippocampus of rats and improve the learning and memory ability of VCI rats.

关 键 词：针刺 脑缺血 认知障碍 脑红蛋白 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]