伤科活血水煎液多组分薄层色谱法鉴别及含量测定  

Research of Shangke Huoxue Decoction based on multi-component TLC identification and content determination

作　　者：周许 王润孔 吴明权 朱力阳[1] 彭伟 涂禾[1] Zhou Xu;Wang Runkong;Wu Mingquan;Zhu Liyang;Peng Wei;Tu He(Department of Traditonal Chinese Medicine Research,Sichuan Province Orthopedic Hospital,Chengdu 610041,China)

机构地区：[1]四川省骨科医院中药研究室,成都610041

出　　处：《国际中医中药杂志》2022年第7期781-785,共5页International Journal of Traditional Chinese Medicine

基　　金：四川省科技厅重点研发项目(2019YFS0541);成都中医药大学“杏林学者”学科人才科研提升计划项目(YYZX2019019);四川省骨科医院科研项目(2019QN03)。

摘　　要：目的建立伤科活血水煎液中阿魏酸、藁本内酯、羟基红花黄色素A和芍药苷4个药效成分薄层色谱法(TLC)鉴别和含量测定的方法,用于其质量评价。方法采用TLC定性鉴别伤科活血水煎液中的阿魏酸、藁本内酯、羟基红花黄色素A和芍药苷,采用HPLC法测定其含量。色谱柱为Waters Symmetry ShieldTM RP18(4.6 mm×250 mm,5μm);流动相为乙腈-0.15%磷酸水溶液,梯度洗脱;流速1.0 ml/min;柱温30℃;检测波长320 nm(33~50 min检测阿魏酸,55~70 min检测藁本内酯)、403 nm(7~31 min检测羟基红花黄色素A)、230 nm(7~31 min检测芍药苷)。结果TLC各斑点清晰;阿魏酸在3.05~48.74μg、藁本内酯在3.50~26.24μg、羟基红花黄色素A在21.34~213.44μg、芍药苷在24.22~193.76μg范围内线性关系良好,方法稳定性、重复性及回收率良好。结论建立了同时测定伤科活血水煎液中4个药效成分的TLC法和HPLC法,适用于伤科活血水煎液的质量评价。Objective To establish the TLC identification method and content determination method of ferulic acid,ligustilide,hydroxysafflor yellow A and paeoniflorin in Shangke Huoxue Decoction for quality evaluation.Methods Ferulic acid,ligustilide,hydroxysafflor yellow A and paeoniflorin were qualitatively identified by TLC method,and the content was determined by HPLC method.Waters Symmetry ShieldTM RP18 column(4.6 mm×250 mm,5μm)was set,the mobile phase consisted of acetonitrile-0.15%phosphoric acid water with gradient elution at a flow of 1.0 ml/min,and the column temperature was 30℃.The detection wavelength was 320 nm(33-50 min for ferulic acid,55-70 min for ligustilide),403 nm(7-31 min for hydroxysafflor yellow A)and 230 nm(7-31 min for paeoniflorin).Results The TLC spots were clear.The linear relationships of ferulic acid,ligustilide,and hydroxysafflor yellow A were good in the range of 3.05-48.74μg,3.50-26.24μg,21.34-213.44μg.The method was stable,repeatable with good recovery rate.Conclusion The TLC and HPLC method for the simutanous determination of the four effective components in Shangke Huoxue Decoction were established,and the methods are suitable for the quality evaluation of Shangke Huoxue Decoction.

关 键 词：色谱法 薄层 色谱法 高压液相 伤科活血水煎液 有效成分 质量控制 

分 类 号：R286.0[医药卫生—中药学]