榆林沙区典型林地不同植被类型对土壤微生物群落结构的影响  

Influence of Different Vegetation Types on Soil Microbial Characteristics of Typical Forest Land in Yulin Sandy Area

作　　者：王岳 丁国栋[3] 刘梦婕[4] 高广磊[3] 于明含[3] 李旭[3] WANG Yue;DING Guo-dong;LIU Meng-jie;GAO Guang-lei;YU Ming-han;LI Xu(School of Resources and Environmental Economics,Inner Mongolia University of Finance and Economics,Hohhol 010070,China;Resource utiliation and environmental protection coordinated development academician expert workstation in the north of China,Inner Mongolia Universiy of Finance and Economics,Inner Mongolia,Hohhot 010070,China;School of Economics and Management,Bejing Forestry University,Bejing 100083,China;School of Soil and Water Conservation,Bejing Forestry University,Beijing 100083,China)

机构地区：[1]内蒙古财经大学资源与环境经济学院,内蒙古呼和浩特010070 [2]祖国北疆资源利用与环境保护协调发展院士专家工作站,内蒙古呼和浩特010070 [3]北京林业大学水土保持学院,北京100083 [4]北京林业大学经济管理学院,北京100083

出　　处：《土壤通报》2022年第4期907-918,共12页Chinese Journal of Soil Science

基　　金：祖国北疆资源利用与环境保护协调发展院士专家工作站开放课题(2021NCDYSZJGZZ-019);内蒙古自然科学基金项目(NO.2020BS03001);国家自然基金项目(No.42061069)资助。

摘　　要：【目的】掌握榆林沙区典型林地土壤微生物特征,明确地上植被对土壤微生物群落结构的影响。【方法】采集榆林沙区四种林分类型土壤,分析其土壤微生物群落结构。【结果】测序共产生有效操作分类单元(OTU)15,509个,各林分间OTU及各类多样性指数没有显著性差异。优势菌种及其丰度土层间变化较大,但林分间优势细菌种一致,丰度排名前五的分别是变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)、厚壁菌门(Firmicutes)和绿弯菌门(Chloroflexi);樟子松、油松林下土壤中,酸杆菌门所占比例最大、分别达到22.32%和29.02%,而在沙柳和小叶锦鸡儿林下土壤中,变形菌门又成为优势菌,比例占到27.64%和28.51%。变形菌门和放线菌门在灌木林土壤中所占比例要高于乔木。【结论】虽然各优势种丰度在林分间略有差别,但差异不显著(P <0.05)。说明在一定区域内微生物群落结构复杂程度是受土壤本底的影响,不同季节或者土壤温度、湿度的变化对微生物群落结构的影响在一段时间后消除,群落结构归于稳定。[Objective] Soil microorganisms play a very important role in reflecting the influence of above-ground vegetation on soil, so the community structure of microorganism is needed to be clarified in soil. [Method] the microbial community structures were analyzed in soils of 4 stands in Yulin Sand Area. [Result] The results showed that Sequencing yielded a total of 15,509 effective operational taxonomic units(OTU) and apparent differences were not observed from the OTUs among stands or the diversity indicators of each unit. Dominant strain species presented large fluctuations in different soil layers, while converged among different stands. Their abundances varied considerably in different soil layers and the top-five dominant species included Proteobacteria, Actinobacteria,Acidobacteria, Firmicutes, and Chloroflexi. In the forest soils of P.sylvestris and P.tabulaeformis, Acidobacteria’s proportion maximized at 22.32% and 29.02% separately. In the forest soils of S.psammophila and C.microphylla,Proteobacteria became dominant and accounted for 27.64% and 28.51%, respectively. Proteobacteria and Actinobacteria owned higher proportions in shrub forest soils than that in arboreal forest soils. [Conclusion] Despite of the slight abundance differences of dominant species among different stands, the differences were not significant(P <0.05).The insignificant difference between forest stands showed that the microbial community structure in a certain area was more affected by the soil background. The influence of different seasons or changes in soil temperature and humidity on the microbial community structure will be eliminated after a period of time, and the community structure will be stable.

关 键 词：榆林沙区 土壤微生物 群落结构 

分 类 号：S182[农业科学—农业基础科学]