苯肽胺酸对低温胁迫下玉米幼苗抗氧化系统和氮代谢的影响  

Effects of N-phenyl-phthalamic Acid on Antioxidant System and Nitrogen Metabolism in Maize Seedlings under Low Temperature Stress

作　　者：杨德光[1] 王琦 何丽 王迎博 孙慧 任静 郑禹琪 宋纯儒 于庆江 翟婧宜 张倩[1] 谢腾龙[1] 李威[1] YANG De-guang;WANG Qi;HE Li;WANG Ying-bo;SUN Hui;REN Jing;ZHENG Yu-qi;SONG Chun-ru;YU Qing-jiang;ZHAI Jing-yi;ZHANG Qian;XIE Teng-long;LI Wei(College of Agronomy,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学农学院,哈尔滨150030

出　　处：《玉米科学》2022年第3期63-71,共9页Journal of Maize Sciences

基　　金：黑龙江省自然科学基金联合引导项目(LH2021C024);东北农业大学“青年才俊”项目(20QC02);农业部种子管理局“绿色品种特性鉴定评价专项”(111721301354052342)。

摘　　要：以玉米品种郑单958为试材,设常温下喷施清水(CK)、常温下喷施苯肽胺酸(PA)、低温下喷施清水(LT)和低温下喷施苯肽胺酸(LT+PA)4个不同处理,低温处理7 d后将玉米幼苗移入常温环境下恢复3 d,测定各处理幼苗生长情况、细胞质膜透性、活性氧(ROS)、抗氧化酶活性和氮代谢相关指标。结果表明,低温胁迫下,幼苗叶片活性氧和丙二醛(MDA)含量显著增加,膜透性增大,胁迫前期会诱导酶促抗氧化防御系统活性增强,随胁迫时间延长,抗氧化酶活性均呈下降趋势;氮素吸收量逐步减少,氮代谢相关酶活性呈明显降低趋势,玉米幼苗生长受到严重抑制。叶面喷施苯肽胺酸可显著减少膜损伤来促进根系对NO_(3)^(-)的吸收和提高抗氧化酶活性,同时氮代谢关键酶活性的上升和GS/GOGAT代谢途径的正常运行在氮代谢中发挥积极作用。Maize variety Zhengdan958 was used as experimental material,and set four different treatments:nor⁃mal temperature water spraying(CK),normal temperature N-phenyl-phthalamic acid spraying(PA),low temperature water spraying(LT)and low temperature N-phenyl-phthalamic acid spraying(LT+PA).After low temperature treat⁃ment for 7 days,the maize seedlings were moved to normal temperature environment to recover for 3 days.The growth,plasma membrane permeability,reactive oxygen species,antioxidant enzyme activity and nitrogen metabo⁃lism of seedlings were measured.The results showed that under low temperature stress,the content of reactive oxy⁃gen species(ROS)and malondialdehyde(MDA)in the leaves of seedlings increased significantly,and the membrane permeability increased.In the early stage of stress,the activity of antioxidant defense system was enhanced,and the activity of antioxidant enzyme decreased with the stress time.Nitrogen absorption decreased gradually,and the ac⁃tivities of enzymes related to nitrogen metabolism decreased significantly,and the growth of maize seedlings was se⁃verely inhibited.Foliar spraying of N-phenyl-phthalamic acid can significantly reduce membrane damage to pro⁃mote the absorption of NO_(3)^(-) by roots and increase the activity of antioxidant enzymes.Meanwhile,the increase in the activity of key enzymes in nitrogen metabolism and the normal operation of GS/GOGAT metabolic pathway play a positive role in nitrogen metabolism.

关 键 词：玉米 苯肽胺酸 低温胁迫 抗氧化系统 

分 类 号：S513.01[农业科学—作物学]