高效液相色谱法测定职业性有害因素尿素的方法确认  

Determination of Occupational Hazard Urea by Liquid Chromatography

作　　者：郭德鹏 李强 GUO De-peng;LI Qiang(Occupational Disease Research Institute of Xishan Coal Electricity Group Co.,Ltd.,Shanxi Taiyuan 030053,China)

机构地区：[1]西山煤电(集团)有限责任公司职业病防治所,山西太原030053

出　　处：《广州化工》2022年第14期129-131,共3页GuangZhou Chemical Industry

摘　　要：通过实验确定检测工作场所空气中职业性有害因素尿素浓度的方法。工作环境中尿素粉尘的采集使用玻璃纤维滤膜,洗脱液洗脱后进样检测,经ODS HYPER Sil(250 mm×4.6 mm,5μm)色谱柱分离,紫外检测器检测。实验表明,尿素浓度在0~200μg/mL范围线性关系良好,相关系数r为0.9996,方法的检出限为0.4μg/mL,采样75 L时,最低检出浓度为0.05 mg/m^(3)。研究表明运用液相色谱仪对空气中尿素样品进行检测,操作简单,结果准确。A liquid chromatography(LC)was developed for the determination of urea in air of workplace.The urea was collected by air samplers with glass fiber filters,then analyzed by HPLC after elution.Samples were separated by LC column ODS HYPER Sil,and detected by UV detector.The qualitative and the quantitative analyses were carried out based on the retention time and peak height or peak area respectively.Urea had a good linear relationship between its content and area within the range of 0~200μg/mL.The correlation coefficent was 0.9996.The detection limit of this method was 0.4μg/mL.The minimum detection concentration of this method was 0.05 mg/m^(3) when sampling 75 L.Urea in air of workplace could be accurately detected easily by liquid chromatography.

关 键 词：液相色谱 尿素 工作场所 职业性有害因素 

分 类 号：R115[医药卫生—公共卫生与预防医学]