Pam_(3)CSK_(4)与黄连素联合用药对小鼠抗MRSA感染的作用  

Anti-MRSA infection by Pam_(3)CSK_(4) and berberine-drug combination in mice

作　　者：彭珊珊 刘思思 巫谢生 孙静 张咏 PENG Shanshan;LIU Sisi;WU Xiesheng;SUN Jing;ZHANG Yong(Jiangxi Academy of Medical Sciences,Nanchang 330000,China;School of Pharmacy,Nanchang University,Nanchang 330036,China)

机构地区：[1]江西省医学科学院,南昌330000 [2]南昌大学药学院,南昌330036

出　　处：《华中师范大学学报（自然科学版）》2022年第4期676-685,共10页Journal of Central China Normal University：Natural Sciences

基　　金：江西省卫生计生委中医药科研课题(2017Z03);江西省卫生计生委科技计划项目(20183002)。

摘　　要：耐药型金黄色葡萄球菌感染的预防和治疗是临床的难题.该文考察了一种新的用药方法,将免疫激动剂Pam_(3)CSK_(4)和黄连的主要抗菌成分黄连素联合使用,通过激活机体先天性免疫反应和中药抗菌两个方面相结合来对抗耐甲氧西林的金黄色葡萄球菌(MRSA).探讨了Pam_(3)CSK_(4)和黄连素在单独或联合使用时,对小鼠受MRSA攻击的保护能力,小鼠对MRSA的清除能力,小鼠感染后分泌IgG和IgM的水平,体外二次感染后淋巴细胞的增殖能力.结果显示Pam_(3)CSK_(4)和黄连素联合使用可以提高小鼠在MRSA致死攻击下的的存活率,增强小鼠对MRSA的清除能力,Pam_(3)CSK_(4)对小鼠的特异性IgG的分泌具有抑制作用,对总IgM的分泌具有促进作用,Pam_(3)CSK_(4)可以促进CD4^(+)和CD8^(+)T细胞以及B细胞在体外的二次增殖,而黄连素可以促进对CD4^(+)T细胞和B细胞的增殖.Prevention and treatment for the infection of methicillin-resistant Staphylococcus aureus(MRSA)is a clinical challenge.To fight against the antibiotic resistance,a new medication was studied in this paper,which is the drug combination of a TLR2 agonist Pam_(3)CSK_(4) and berberine.The combination was able to defend against MRSA through two ways,activation of immunity and antibacterial effect of Chinese traditional medicine.The survival,MRSA clearance and secretion of specific IgG and overall IgM of mice were investigated under MRSA attack when the two drugs were treated alone or together.The secondary proliferation of lymphocytes in vitro was also detected to study the influence of drugs.The results suggested that the combination of Pam_(3)CSK_(4) and berberine would improve the survival rate of mice under lethal MRSA attack,and enhance the ability of MRSA clearance.Pam_(3)CSK_(4) was able to suppress the secretion of MRSA-specific IgG in mice,while stimulate the secretion of overall IgM.The secondary proliferation of CD4^(+),CD8^(+)T cells and B cells were also stimulated in vitro.As for berberine,the proliferation of CD4^(+)T cells and B cells in vitro were promoted.

关 键 词：耐甲氧西林的金黄色葡萄球菌 Pam_(3)CSK_(4) 黄连素 

分 类 号：R915[医药卫生—微生物与生化药学]