在不同土壤体系下漆酶催化降解对乙酰氨基酚的差异研究  

Effects of laccase degradation on acetaminophen in different soil systems

作　　者：龚志敏 王佳豪 刘鹏[1] 高士祥[1] GONG Zhimin;WANG Jiahao;LIU Peng;GAO Shixiang(State Key Laboratory of Pollution Control and Resource Reuse,School of the Environment,Nanjing University,Nanjing,210046,China)

机构地区：[1]南京大学环境学院,污染控制与资源化研究国家重点实验室,南京210046

出　　处：《环境化学》2022年第7期2256-2263,共8页Environmental Chemistry

基　　金：国家重点研发专项(2019YFC1805700)资助

摘　　要：漆酶广泛分布在天然环境中,可以有效催化降解水体和土壤中有机污染物。对乙酰氨基酚(Acetaminophen,AAP)是一种最常用的止痛和退烧药物,环境残留浓度较高,AAP及其转换产物均易导致肝脏和肾脏的损害。研究表明,在水体介质中漆酶可以有效降解AAP,但是对于土壤介质中漆酶降解AAP的研究却较少。本文系统地研究了不同地区土壤(江苏宜兴水稻土、陕西洛川黑垆土、江西鹰潭红土和广东德庆砖红土)体系下漆酶降解AAP的差异,包括其对AAP的吸附作用和对漆酶的酶活影响。结果表明,组成成分淤泥含量较高的江苏宜兴水稻土和陕西洛川黑垆土对AAP的吸附性能较大,平衡吸附量分别为12.03 mg·g^(−1)和28.37 mg·g^(−1),而江西鹰潭红土和广东德庆砖红土对AAP无明显吸附。江西鹰潭红壤土介质中,酶的活性是最稳定的,同时在该土壤介质中漆酶对AAP的降解效果优异,而陕西洛川由于土壤介质pH值呈弱碱性,在该pH条件下漆酶的活性较低,因此对于污染物AAP的降解效率也较低。江苏宜兴水稻土介质中的酶活相对广东德庆砖红土较高,但是土壤介质中SOM的含量较多,综合在降解效果与广东德庆砖红土接近。本研究为评价实际土壤介质对漆酶降解环境污染物提供科学依据。Laccase is widely distributed in the environment and can effectively degrade persistent pollutants in water.Acetaminophen(AAP)is one of the most commonly prescribed over-the-counter drugs to relieve pain and reduce fever,with high environmental residual concentrations.Both AAP and its conversion products are likely to affect human health.Previous studies have shown that laccase can effectively degrade AAP in aqueous media,but there are few studies on laccase degradation of AAP in soil media.This study systematically investigated the effects of soils from different site on the laccase-catalyzed removal of AAP,including the adsorption and enzyme activity.Four different soils were sampled from Yixing paddy soil in Jiangsu,Luochuan black soil in Shaanxi,Yingtan red soil in Jiangxi,and Deqing brick red soil in Guangdong.The results showed that Jiangsu Yixing paddy soil and Luochuan black soil in Shaanxi had higher adsorption capacity for AAP,with saturated adsorption capacities of 12.03 mg·g^(−1) and 28.37 mg·g^(−1),respectively.Yingtan red soil in Jiangxi and brick red soil in Deqing,Guangdong did not significantly adsorb AAP.The enzyme activity is the most stable in Yingtan red soil.At the same time,the degradation effect of laccase on AAP is excellent in this soil.In Luochuan black soil,the activity of laccase was low because the pH value was weakly alkaline,so the degradation efficiency of pollutant AAP was also low.However,the enzyme activity in the Yixing paddy soil in Jiangsu is relatively high,but the content of SOM in soil was high,so the degradation effect was similar to that in Deqing brick red soil.This study provides a scientific basis for evaluating soil for laccase degradation of environmental pollutants.

关 键 词：漆酶 土壤 对乙酰氨基酚 降解 吸附 

分 类 号：X53[环境科学与工程—环境工程]