大麻二酚上调BDNF、突触蛋白发挥抗抑郁作用  

Cannabidiol up-regulates BDNF and synaptic protein to exert antidepressant effects

作　　者：杨艳 马滕滕 边宇婧 顾江娜 孙毓媛 温朕勇 谢建平 袁云 郭英 Yang Yan;Ma Tengteng;Bian Yujng;Gu Jiangna;Sun Yuyuan;Wen Zhenyong;Xie Jianping;Yuan Yun;Guo Ying(School of Basic Medical Sciences,Kunming Medical University,Kunming 650000;Library,Yunnan Minzu University,Kunming 650000)

机构地区：[1]昆明医科大学基础医学院,昆明650000 [2]云南民族大学图书馆,昆明650000

出　　处：《安徽医科大学学报》2022年第8期1206-1210,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金地区科学基金项目(编号:82060650、31760297);云南省科技计划项目基础研究专项面上项目(编号:202001AT070137);云南省基础研究计划项目[编号:2018FE001(-189)]。

摘　　要：目的研究大麻二酚(CBD)对抑郁样小鼠的快速抗抑郁作用及可能的作用机制。方法采用慢性束缚建立小鼠抑郁模型。将供试小鼠分为5组:正常对照组、模型组、阳性对照组、CBD低剂量组(25 mg/kg)、CBD高剂量组(50 mg/kg)。在行为学实验前1 h对每组小鼠灌胃给药。行为学实验结束后,收集海马和前额皮层样本,采用ELISA测定脑源神经营养因子(BDNF)、突触后密度-95蛋白(PSD-95)、突触素(SYP)、雷帕霉素靶蛋白(mTOR)。结果与模型组相比,CBD低剂量组旷场实验中央区域活动路程百分比、中央区域活动时间百分比和总路程均增加。与模型组相比,CBD低剂量组强迫游泳实验不动时间百分比降低。ELISA检测结果显示,CBD能快速上调前额皮层中BDNF、PSD-95浓度,快速上调海马和前额皮层中SYP、mTOR浓度。结论CBD能快速改善抑郁样小鼠的行为学表现,快速上调海马或前额皮层中的BDNF、突触蛋白水平。Objective To study the rapid antidepressant effects of cannabioiol(CBD)on depression-like mice and its possible mechanism.Methods Chronic restraint was used to establish a mouse depression model.The test mice were divided into 5 groups:normal control group,model group,positive control group,CBD low-dose group(25 mg/kg)and CBD high-dose group(50 mg/kg).The mice in each group were given intragastric administration one hour before the behavioral experiment.After the behavioral experiment,the hippocampus and prefrontal cortex specimens were collected,and brain-derived neurotrophic factor(BDNF),postsynaptic density protein 95(PSD-95),synaptophysin(SYP)and target of rapamycin(mTOR)were tested by ELISA.Results Compared with the model group,the central area activity distance percentage,the central area activity time percentage and total distance increased in the open field experiment in the CBD low-dose group.Compared with the model group,the percentage of immobility in the forced swimming experiment in the low-dose CBD group decreased.The ELISA test results showed that CBD could rapidly increase the concentration of BDNF and PSD-95 in the prefrontal cortex,as well as the concentration of SYP and mTOR in the hippocampus and prefrontal cortex.Conclusion CBD can rapidly improve the behavioral performance of depression-like mice,and rapidly up-regulate the level of BDNF and synaptic protein in the hippocampus or prefrontal cortex.

关 键 词：大麻二酚 抗抑郁作用 前额皮质 海马 

分 类 号：R964[医药卫生—药理学]