SOD拟似剂Tempol改善间歇性低氧小鼠主动脉舒张功能  

SOD mimetic Tempol improves aortic vasodilation function in mice exposed to intermittent hypoxia

作　　者：蒋倩 圣豪 刘国莹 余孝海[1] 钟明奎[1] Jiang Qian;Sheng Hao;Liu Guoying;Yu Xiaohai;Zhong Mingkui(Dept of Physiology,Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学生理学教研室,合肥230032

出　　处：《安徽医科大学学报》2022年第8期1279-1282,1288,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81070066);安徽省教育厅自然科学重点项目(编号:KJ2018A0174);安徽医科大学校科研基金(编号:2019xkj009)。

摘　　要：目的探索超氧化物歧化酶(SOD)拟似剂四甲基哌啶(Tempol)对间歇性低氧小鼠血管舒张功能的影响。方法将雄性C57小鼠随机分为正常氧(Control)组、间歇性低氧(IH)组、间歇性低氧Tempol干预(IH+Tempol)组。IH组和IH+Tempol组小鼠放入低氧仓进行间歇性低氧处理,每天8 h,连续4周。IH+Tempol组在间歇性低氧处理最后一周每天给予Tempol(100 mg/kg)灌胃。离体血管张力测定系统检测血管的功能,荧光探测针-二氢乙啶(DHE)染色法检测血管活性氧(ROS)含量,ELISA法测量一氧化氮(NO)浓度。结果与Control组相比,间歇性低氧4周后,IH组小鼠胸主动脉对乙酰胆碱(ACh)引起的内皮依赖性舒张功能下降、心肌肥大,胸主动脉中ROS增加而NO水平降低;应用Tempol可使IH小鼠胸主动脉中ROS水平降低、NO浓度增加,改善间歇性低氧引起的内皮依赖性舒张功能受损和心肌肥大。结论Tempol可能通过降低ROS,增加NO的生物利用度,改善间歇性低氧引起的心血管功能障碍。Objective To investigate the effect of superoxide dismutase(SOD)mimetic 4-Hydroxy-TEMPO(Tempol)on aortic vasodilation function in mice exposed to intermittent hypoxia.Methods Male C57 mice were randomly divided into normal oxygen(Control)group,intermittent hypoxia(IH)and IH+Tempol group.The mice in IH and IH+Tempol group were exposed to 4-weeks intermittent hypoxia,8 h daily.In IH+Tempol treatment group,the mice were given Tempol(100 mg/kg)each day by the oral administration in the last week of intermittent hypoxia.The isolated thoracic aorta reactivity was studied using wire myography,the content of reactive oxygen species(ROS)was detected by dihydroethidium(DHE)staining,the concentration of nitric oxide(NO)was measured by ELISA kits.Results Compared with Control group,the endothelium-dependent vasodilation of thoracic aorta induced by acetylcholine(ACh)significantly decreased and cardiac hypertrophy,the level of aortic ROS significantly increased,the concentration of aortic NO was significantly reduced in the IH group mice.Treatment with Tempol could significantly improve the aortic endothelium-dependent vasodilation dysfunction and cardiac hypertrophy,and significantly inhibited the increase of ROS and decrease of NO in the thoracic aorta induced by intermittent hypoxia.Conclusion Tempol can improve intermittent hypoxia induced-cardiovascular dysfunction in mice by reducing ROS and increasing the bioavailability of NO.

关 键 词：间歇性低氧 氧化应激 内皮功能障碍 TEMPOL 

分 类 号：R331.36[医药卫生—人体生理学]