miRNA及细胞周期调节因子在垂体腺瘤侵袭性和非侵袭性组织中的表达差异性研究  

The study of differential expression of miRNA and cell cycle regulators in invasive and non-invasive tissues of pituitary adenomas

作　　者：田郡 韩中保 杨留才 滕飞翔 张虎 TIAN Jun;HAN Zhong-bao;YANG Liu-cai;TENG Fei-xiang;ZHANG Hu(Jiangsu Vocational College of Medicine,Yancheng 224000,Jiangsu,China)

机构地区：[1]江苏医药职业学院,江苏盐城224000

出　　处：《中国病原生物学杂志》2022年第7期774-778,共5页Journal of Pathogen Biology

摘　　要：目的验证几种敏感微小核糖核酸(miRNA137、miRNA199a)及细胞周期因子在垂体腺瘤的侵袭性和非侵袭性组织中的表达差异。方法选取2016年1月-2021年12月入住的垂体瘤病患者85例,其中侵袭性垂体瘤38例,非侵袭性垂体瘤47例。qRT-PCR验证两组患者的垂体瘤组织miRNA含量的表达差异性,通过免疫组化和qRT-PCR等技术检测两组患者瘤体组织相关指标的差异表达。结果侵袭性垂体瘤组织和非侵袭性垂体瘤组织中miRNA137相对表达量为分别为2.95±0.11和1.30±0.08,差异具有统计学意义(F=7.838,P<0.05);miRNA199a相对表达量分别为0.45±0.03和0.96±0.09,差异具有统计学意义(F=41.031,P<0.05)。转染48 h后,过载组、空载组和抑制组miRNA137相对表达量分别为4.92±0.18、2.74±0.09和0.85±0.04,miRNA199相对表达量分别为3.79±0.14、1.93±0.08和0.72±0.06,组间比较差异均有统计学意义(P<0.05)。侵袭性垂体瘤组织中P21和P27阳性率分别为31.58%和36.84%,非侵袭性垂体瘤组织中P21和P27阳性率分别为36.17%和40.43%,组间比较差异均无统计学意义(P>0.05)。结论不同组垂体腺瘤(侵袭或非侵袭)组织中miRNA137、199和P21、P27的表达存在差异,可作为临床检测生物标志物,对识别侵袭性垂体瘤,增加药物治疗敏感性及作为治疗垂体瘤的新型靶向药物提供了参考依据。Objective To verify the expression differences of several sensitive miRNAs(137,199 a)and cell cycle factors in invasive and non-invasive groups of pituitary adenomas.Methods 85 patients with pituitary adenoma were selected from January 2016 to December 2021,including 38 invasive pituitary adenomas and 47 non-invasive pituitary adenomas.qRT-PCR was used to verify the expression difference of miRNA content among different groups,and the differential expression of tumor tissue related indexes between the two groups was detected by immunohistochemistry and qRT-PCR.Results The relative expression of miRNA137 in invasive and non-invasive pituitary tumors was(2.95±0.11)and(1.30±0.08),and the difference between the two groups was statistically significant(F=7.838,P<0.05);The relative expression of miRNA199 a in invasive and non-invasive pituitary tumors were(0.45±0.03)and(0.96±0.09),the difference between the two groups was statistically significant(F=41.031,P<0.05).After 48 hours of transfection,the relative expression of miRNA137 in overload group,no load group and inhibition group was(4.92±0.18),(2.74±0.09)and(0.85±0.04).The relative expression of miRNA199 in overload group,no-load group and inhibition group was(3.79±0.14),(1.93±0.08)and(0.72±0.06),There was significant difference between the two groups(P<0.05).The positive rates of P21 and P27 were 31.58%and 36.84%,respectively.The positive rates of P21 and P27 were 36.17%and 40.43%,There was no significant difference between groups(P>0.05).Conclusion The expression of miRNA137,199 a and P21,P27 were different in different groups of pituitary adenomas(invasive or non-invasive).Using these sensitive indicators as biomarkers for clinical detection can provide a strong basis for identifying invasive pituitary tumors,increasing drug sensitivity and being a new targeted drug for the treatment of pituitary tumors.

关 键 词：微小核糖核酸 细胞周期调节因子 垂体腺瘤 

分 类 号：R37[医药卫生—病原生物学]