贵州省一起布鲁氏菌病聚集性疫情的病原学调查与分子流行病学分析  

Etiological investigation and molecular epidemiology of an aggregated epidemic of brucellosis in Guizhou Province

作　　者：谭勤琴 王月[2] 刘英[2] 陶忠发 杨幸贵 马青[2] 胡勇[1] 李世军[1,2] TAN Qin-qin;WANG Yue;LIU Ying;TAO Zhong-fa;YANG Xing-gui;MA Qing;HU Yong;LIShi-jun(School of Ppublic Hhealth,the Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Educatin,Guizhou Medical University,Guiyang,GuiZhou 550025,China;Guizhou provincial Center for Disease Control and Prevention)

机构地区：[1]贵州医科大学公共卫生与健康学院,环境污染与疾病监控教育部重点实验室,贵州贵阳550025 [2]贵州省疾病预防控制中心

出　　处：《中国病原生物学杂志》2022年第7期802-806,共5页Journal of Pathogen Biology

基　　金：贵州省高层次创新型人才培养项目(黔科合[2016]4021);贵州省传染病人才培养基地建设项目子项目(No.RCJD2102)。

摘　　要：目的对2021年贵州省一起疑似布鲁氏菌病聚集性疫情开展病原学调查和分子流行病学分析。方法采用血培养法从疑似感染布鲁氏菌病的患者和病羊的血液分离布鲁氏菌,分别运用BCSP31-PCR和AMOS-PCR对疑似菌株进行布鲁氏菌属及种/型的鉴定,应用多位点可变数目串联重复序列(MLVA-16)分析技术对其进行分子分型,将MLVA分型结果与近年来贵州省及国内各地布鲁氏菌代表株进行聚类分析,了解菌株间的聚类关系。结果从16份疑似患者全血标本分离出5株疑似布鲁氏菌,从19份疑似病羊全血标分离出1株疑似布鲁氏菌,BCSP31-PCR将6株疑似菌均鉴定为布鲁氏菌属细菌,AMOS-PCR其均鉴定为羊种布鲁氏菌。MLVA-16分析显示6株疫情菌株被分为3种MLVA型,其中来自患者的分离株GZ-QN 1、GZ-QN 4及GZ-QN 6共享MLVA型1(1-5-3-13-2-2-3-2-4-20-8-7-4-3-4-6),来自1患者分离株GZ-QN 8与来自1病羊分离株株GZ-QN A691共享MLVA型2(1-5-3-13-2-2-3-2-4-20-8-8-4-3-6-6),来自1患者分离株GZ-QN 14为独立的MLVA型3(1-5-3-13-2-2-3-2-4-20-8-8-4-3-7-6)。基于MLVA-16分型数据聚类显示,引起贵州省本起疫情的布鲁氏菌与羊种生物3型菌株聚类较近,且与来自福建、新疆的布鲁氏菌聚在同一小分支。结论引起本起疫情病原体为的羊种布鲁氏菌且具有MLVA型别多样性,可能为与福建、新疆等地的羊种布鲁氏菌菌株具有关联的输入性感染引起的聚集性疫情。Objective To provide etiological basis for case diagnosis and epidemic prevention and control,Pathogenic investigation and molecular epidemiological analysis were carried out on a suspected brucellosis epidemic an aggregated epidemic of suspected brucellosis in Guizhou Province in 2021 was performed by etiological investigation and molecular epidemiological analysisin Guizhou Province in 2021.Methods After the Brucella spp.strains were isolated from the blood samples of suspected infected patients and goats by blood culture method,they were further identified by genus and species/type using BCSP31-PCR and AMOS-PCR respectively,and also typed using multiple locus variable-number tandem repeat analysis(MLVA-16).To analyze the clustering relationships of different strains,the MLVA typing results of Brucella spp.strains in Guizhou province were clustered with representative strains of Brucella spp.from other regions of China in recent years.In order to analyzed the clustering relationship in different strains,the results of MLVA typing were clustered with representative strains of Brucella spp.in Guizhou province and other regions of China in recent years.Results Six suspected Brucella spp.strains were isolated,including 5 isolates from 16 whole blood samples of suspected patients and 1 isolate from 19 whole blood samples of suspected sick goats,which were identified as Brucella by BCSP31-PCR melitensis(B.melitensis)by BCSP31-PCR and melitensis Brucella(B.melitensis)by BCSP31-and AMOS-PCR assays.MLVA-16 analysis showed that the six strains were divided into three MLVA types,among which three strains isolated from different patients(GZ-QN 1,GZ-QN 4 and,GZ-QN 6)shared MLVA type 1(1-5-3-13-2-2-3-2-2-4-20-8-7-4-3-4-6),the one patient strain(GZ-QN 8)(GZ-QN 8 isolated from a patient)and one sick goat strain(GZ-QN A691)(GZ-QN A691 isolated from a sick sheep)shared MLVA type 2(1-5-3-13-2-3-2-4-20-8-4-3-6-6),and another one patient strainisolate(GZ-QN 14)(GZ-QN 14)isolated from the patient was an independent MLVA type 3(1-5-3

关 键 词：布鲁氏菌病 布鲁氏菌 AMOS-PCR MLVA 贵州省 

分 类 号：R378.5[医药卫生—病原生物学]