禽流感油乳剂灭活疫苗抗原质量快速评价方法  

Rapid Evaluation Method for the Antigen Quality of Avian Influenza Oil Emulsion Inactivated Vaccine

作　　者：赖汉漳 王小芬 麦康聪 盘伟岚 陈培军 陈华坚 陈瑞爱[1,2] LAI Hanzhang;WANG Xiaofen;PAN Weilan;CHEN Peijun;CHEN Huajian;XU Jiahua;CHEN Ruiai(College of Veterinary Medicine,South China Agricultural University,Guangzhou Guangdong 510642;Guangdong Enterprise Key Laboratory of Biotechnology R&D of Veterinary Biologics,Key Laboratory of Biotechnology and Bioproducts Development for Animal Epidemic Prevention,Ministry of Agriculture,Zhaoqing Dahuanong Biological Medicine Co.,Ltd.,Zhaoqing Guangdong 526238)

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]肇庆大华农生物药品有限公司,农业部动物疫病防控生物技术与制品创制重点实验室,广东省兽用生物制品技术研究与应用企业重点实验室,广东肇庆526238

出　　处：《广东畜牧兽医科技》2022年第4期21-23,共3页Guangdong Journal of Animal and Veterinary Science

摘　　要：本研究建立了基于肉豆蔻酸异丙酯作为破乳剂的一种禽流感油乳剂疫苗抗原快速检测方法,该方法成本低且环境友好,实验条件简易,保真度高,可在数小时内准确快速测定禽流感等油乳剂疫苗成品抗原HA效价及分析其抗原性差异。使用该方法能够方便广大养殖用户从不同来源疫苗中有针对性地选择优质高效的疫苗,并在使用疫苗前确定其抗原效价,避免使用低效价疫苗,确保预期防控效果,同时也方便兽药监察部门对市场上各个厂家的禽流感油乳剂灭活疫苗的质量进行实时监测。A rapid evaluation method for avian influenza oil emulsion vaccine antigen based on isopropyl myristate as a demulsifier was established,which is low cost and environmentally friendly,and the experimental conditions are simple. It can accurately and quickly determine the HA titer of finished oil emulsion vaccines such as avian influenza and analyse their antigenic differences within a few hours. The method can facilitate the selection of high quality and efficient vaccines from different sources and the determination of their antigenic potency before use,avoiding the use of low potency vaccines and ensuring the expected control effect.

关 键 词：禽流感疫苗 抗原质量 评价方法 

分 类 号：S852.657[农业科学—基础兽医学]