基于“调气解毒”理论探讨肺瘤平膏调控脂质代谢逆转肿瘤相关树突状细胞功能的机制研究  

Mechanism of Feiliuping Ointment in Regulating Lipid Metabolism and Reversing the Function of Tumor-related Dendritic Cells Based on the Theory of Regulating Qi and Removing Toxin

作　　者：张曦文[1] 栾美琪 席玉棚 郑红刚[1] 花宝金[1] ZHANG Xiwen;LUAN Meiqi;XI Yupeng;ZHENG Honggang;HUA Baojin(Guang'anmen Hospital,Chinese Academy of Chinese Medical Sciences,Beijing 100053,China;School of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]中国中医科学院广安门医院,北京100053 [2]黑龙江中医药大学药学院,哈尔滨150040

出　　处：《世界中医药》2022年第11期1528-1534,1539,共8页World Chinese Medicine

基　　金：中国中医科学院科技创新工程项目(CI2021B009);国家中医药管理局中医药创新团队项目(ZYYCXTD-C-202205)。

摘　　要：目的:体外模拟肿瘤微环境,构建瘤相关树突状细胞(TDCs)模型,探究肺瘤平膏含药血清改善脂质堆积,逆转TDCs功能的效应机制。方法:制备肺瘤平膏及空白含药血清,通过分离、诱导并与路易斯(Lewis)肺癌细胞共培养获得TDCs,通过流式细胞仪检测肺瘤平膏干预下的TDCs的脂质含量和表型变化,基于Luminex技术定量检测TDCs白细胞介素-12p70、γ干扰素的分泌;构建TDCs与T细胞共培养体系,探究肺瘤平膏含药血清对TDCs调节混合淋巴细胞增殖和T细胞亚群的影响。结果:肺瘤平膏含药血清作用后的TDCs,脂质含量小于空白血清对照组(P<0.01),CD80、CD86表达水平高于空白血清组(P<0.05),白细胞介素-12p70、γ干扰素的分泌高于空白血清组(P<0.05);肺瘤平膏含药血清作用后的混合细胞增殖能力较空白血清增强(P<0.05),肺瘤平膏含药血清能够提高干预后TDCs与T细胞共培养CD4^(+)、CD8^(+)表达比例(P<0.05),降低调节性T细胞的表达。结论:肺瘤平膏含药血清能够有效地降低TDCs中的脂质堆积,促进TDCs的成熟和抗原呈递。Objective:To explore the mechanism of Feiliuping Ointment in improving lipid accumulation and reversing the function of tumor-related dendritic cells(TDCs)by simulating tumor microenvironment in vitro and constructing TDCs model.Methods:Serum with and without Feiliuping Ointment was prepared,and lung TDCs were obtained by isolation,induction and co-culture with Lewis lung cancer cells.Flow cytometry was used to detect the lipid content and phenotypic changes of TDCs under the intervention of Feiliuping Ointment.Luminex technology was employed to quantitatively detect the secretion of TDCs cytokines IL-12p70 and IFN-γ.The co-culture system of TDCs and T cells was constructed to explore the effects of Feiliuping Ointment on TDCs'regulation of mixed lymphocyte proliferation and T cell subsets.Results:The lipid content in the TDCs treated with Feiliuping Ointment was lower(P<0.01),and the expression levels of CD80 and CD86 and the secretion of IL-12P70 and IFN-γ were higher(P<0.05,P<0.05)than those of the blank serum control group.In addition,compared with the conditions in the blank serum,the proliferation ability of mixed cells and the expression proportion of CD4^(+)and CD8^(+)in the co-culture of TDCs and T cells were enhanced(P<0.05,P<0.05),and the expression of Tregs cells was reduced(P<0.05)after treatment with serum containing Feiliuping Ointment.Conclusion:Feiliuping Ointment could reduce the lipid accumulation in TDCs and promote the maturation and antigen presentation ability of TDCs.

关 键 词：调气解毒 肺癌 肺瘤平膏 肿瘤微环境 肿瘤相关树突状细胞 脂代谢 T细胞 

分 类 号：R273[医药卫生—中西医结合]