Establishment and Characterization of a Fin Cell Line Derived from the Atlantic Salmon Salmo salar and Its Application to Fish Virology Study  

作　　者：JIA Peng LIN Lirong XU La YI Meisheng JIA Kuntong 

机构地区：[1]School of Marine Sciences,Sun Yat-sen University,Guangzhou 510000,China [2]Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai),Zhuhai 519082,China [3]Zhuhai Key Laboratory of Marine Bioresources and Environment,Sun Yat-sen University,Guangzhou 510000,China [4]Marine Biology Institute of Shandong Province,Shandong Key Laboratory of Disease Control in Mariculture,Qingdao 266104,China

出　　处：《Journal of Ocean University of China》2022年第4期946-954,共9页中国海洋大学学报（英文版）

基　　金：supported by the China Postdoctoral Science Foundation (No. 2019M653152);the Pearl River S&T Nova Program of Guangzhou (No. 201806010047);the National Natural Science Foundation of China (No. 31771587);Fundamental Research Funds for the Central Universities (No. 19lgpy102);the Natural Science Foundation of Guangdong Province (No. 2019A1515110842)

摘　　要：Atlantic salmon(Salmo salar)is an important economic fish that is seriously threatened by various viruses.A cell line designated as ASF derived from the caudal fin tissue of Atlantic salmon was established and characterized in this study.ASF cells grew well in Dulbecco’s modified Eagle’s medium(DMEM)containing 20%fetal bovine serum at 20℃.DNA sequencing and comparative analysis of the cytochrome B gene verified that the ASF cell line originated from Atlantic salmon.Chromosome analysis indicated that the modal chromosome number of ASF cells was 58.Viral susceptibility test showed that ASF cells were susceptive to two important fish viruses,viral hemorrhagic septicemia virus(VHSV)and red-spotted grouper nervous necrosis virus(RGNNV).Viral replication in ASF cells was further confirmed by qRT-PCR and transmission electron microscopy.Moreover,VHSV and RGNNV infections could induce the cellular responses in ASF cells,as indicated by the differential expression of cellular antiviral response-related genes,interferon-1 and Mx-1.In conclusion,the newly established ASF cell line can be applied as an in vitro tool in fish virology and immunity studies.

关 键 词：Salmo salar cell line viral hemorrhagic septicemia virus nervous necrosis virus immune response 

分 类 号：S943[农业科学—水产养殖]