类风湿关节炎患者滑膜组织中GLUT1表达及临床意义  

The Expression and Clinical Significance of GLUT1 in SynovialTissue of Patients with Rheumatoid Arthritis

作　　者：孙敬波 刘冰[1] 杨丽华[1] 袁红亮 刘建瑞[2] SUN Jingbo;LIU Bing;YANG Lihua;YUAN Hongliang;LIU Jianrui(Department of Clinical Laboratory,Qinhuangdao Hospital of TraditionalChinese Medicine,Qinhuangdao 066000,China;Department of Science and Education,Qinhuangdao Hospital of TraditionalChinese Medicine,Qinhuangdao 066000,China)

机构地区：[1]秦皇岛市中医医院检验科,河北秦皇岛066000 [2]秦皇岛市中医医院科教科,河北秦皇岛066000

出　　处：《标记免疫分析与临床》2022年第7期1127-1130,1134,共5页Labeled Immunoassays and Clinical Medicine

基　　金：秦皇岛市科学技术局项目(雷公藤多苷等药物治疗类风湿性关节炎过程中多种监测指标变化规律的研究)(编号:201902A066)。

摘　　要：目的探究类风湿关节炎(RA)患者滑膜组织中葡萄糖转运蛋白1(GLUT1)表达水平及意义。方法选取2019年1月至2020年12月本院收治的110例RA患者为RA组,另纳入同期65例半月板损伤者为对照组。实时荧光定量PCR(qRT-PCR)法检测滑膜组织GLUT1 mRNA、低氧诱导因子-1α(HIF-1α)mRNA表达水平;免疫比浊法测定血清类风湿因子(RF)水平;魏氏法测定红细胞沉降率(ESR);对RA患者进行28个关节疾病活动(DAS28)评分;比较缓解组、活动组RA患者滑膜组织GLUT1 mRNA、HIF-1αmRNA、ESR及血清RF水平;Pearson法分析活动期RA患者滑膜组织GLUT1 mRNA、HIF-1αmRNA表达水平与ESR、血清RF水平及DAS28评分的相关性,及GLUT1 mRNA表达水平与HIF-1αmRNA的相关性。结果RA组患者滑膜组织GLUT1 mRNA、HIF-1αmRNA、ESR、血清RF水平均高于对照组(t=11.464、10.212、15.503、22.999,P<0.05);活动组RA患者滑膜组织GLUT1 mRNA、HIF-1αmRNA、ESR、血清RF水平、DAS28评分高于缓解组(t=3.079、3.526、4.914、9.897、15.822,P<0.05);活动期RA患者滑膜组织GLUT1 mRNA、HIF-1αmRNA表达水平与ESR、血清RF水平及DAS28评分均呈正相关(P<0.05),GLUT1 mRNA表达水平与HIF-1αmRNA呈正相关(r=0.545,P<0.05)。结论RA患者滑膜组织GLUT1 mRNA表达水平较高,与HIF-1αmRNA、RF、ESR及疾病活动度显著相关,其可能是诊治RA的潜在靶标。Objective To explore the expression level and significance of glucose transporter protein 1(GLUT1)in synovial tissue of patients with rheumatoid arthritis(RA).Methods A total of 110 RA patients admitted to our hospital from January,2019 to December,2020 were selected as the RA group,while 65 patients with meniscal injury during the same period were included as the control group.Real-time fluorescent quantitative PCR(qRT-PCR)method was used to detect expression levels of GLUT1 mRNA and hypoxia inducible factor-1α(HIF-1α)mRNA in synovial tissue;immunoturbidimetric method was applied to determinate the level of serum rheumatoid factor(RF);westergren method was used to measure erythrocyte sedimentation rate(ESR);28-joint disease activity(DAS28)scores was performed on the RA patients;levels of GLUT1 mRNA,HIF-1αmRNA,ESR and serum RF in the synovial tissue of RA patients in the remission group and the active group were then compared;Pearson analysis was used to evaluate the correlation between GLUT1 mRNA,HIF-1αmRNA expression levels with ESR,serum RF levels and DAS28 scores in synovial tissues of patients with active RA,and the correlation between GLUT1 mRNA expression level with HIF-1αmRNA.Results Levels of GLUT1 mRNA,HIF-1αmRNA,ESR,and serum RF of synovial tissue in RA group were higher than those in the control group(t=11.464,10.212,15.503,22.999,P<0.05);synovial tissue GLUT1 mRNA,HIF-1αmRNA,ESR,serum RF,and DAS28 scores of RA patients in the active group were higher than those in the remission group(t=3.079,3.526,4.914,9.897,15.822,P<0.05);expression levels of GLUT1 mRNA and HIF-1αmRNA in synovial tissues of patients with active RA were positively correlated with ESR,serum RF level and DAS28 scores(P<0.05),the expression level of GLUT1 mRNA was positively correlated with HIF-1αmRNA(r=0.545,P<0.05).Conclusion The expression level of GLUT1 mRNA in synovial tissues of RA patients is high,and it is significantly related to HIF-1αmRNA,RF,ESR and disease activity,which could serve as a potential target for the d

关 键 词：葡萄糖转运蛋白1 类风湿关节炎 滑膜组织 低氧诱导因子-1Α 

分 类 号：R593.22[医药卫生—内科学]