黄瓜雄性不育突变体C0128的鉴定与不育基因的初步定位  

Identification of Male Sterile Mutant C0128 and Preliminary Mapping of MS-4 Gene in Cucumber

作　　者：杨志敏 岳宏忠[2] 张海强 李玉红[1] YANG Zhimin;YUE Hongzhong;ZHANG Haiqiang;LI Yuhong(College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China;Vegetable Research Institute of Gansu Academy of Agricultural Sciences,Lanzhou 730070,China)

机构地区：[1]西北农林科技大学园艺学院,陕西杨陵712100 [2]甘肃农业科学院蔬菜研究所,兰州730070

出　　处：《西北植物学报》2022年第6期901-908,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31860557)。

摘　　要：为丰富黄瓜雄性不育基因库及雄性不育理论研究,该研究以1个性状稳定遗传的黄瓜雄性不育突变体C0128为材料,对其进行表型鉴定、遗传分析和雄性不育基因初步定位。结果表明:(1)突变体C0128的雄花花瓣和花药均较小,且花药上无花粉粒产生。(2)遗传规律分析发现,突变体C0128的雄性不育性状受1对隐性核基因MS-4调控。(3)利用来自Gy14×C0128 F_(2)群体的1231个单株及12个多态性的SSR分子标记,最终将MS-4初步定位在黄瓜Chr3上遗传距离为3.7 cM、物理距离为1.21 Mb的区间内。研究证实,C0128为一个新的黄瓜雄性不育突变体,MS-4的初步定位结果可为进一步克隆目的基因、研究雄性不育机制奠定基础。In order to enrich the gene pool and the theoretical research of male sterility in cucumber,a male sterility mutant C0128 was used as material for phenotypic identification,genetic analysis and preliminary mapping of male sterility gene.The results showed that:(1)the male petals and anthers of mutant C0128 were smaller than that of wild type CCMC,and no pollen grains were produced on the anthers.(2)Genetic analysis showed that the male sterility of the mutant was controlled by a recessive nuclear gene MS-4.(3)Using Gy14×C0128 F_(2)population with 1231 plants and 12 polymorphic SSR markers,the MS-4 gene was initially located on cucumber Chr3,and the genetic distance and physical distance was 3.7 cM and 1.21 Mb,respectively.This study confirmed that C0128 is a new male sterile mutant in cucumber.The preliminary mapping results of MS-4 can lay a foundation for further cloning of target gene and studying the mechanism of male sterility in C0128.

关 键 词：黄瓜 雄性不育 突变体 MS-4 初步定位 

分 类 号：Q319.3[生物学—遗传学] Q786[农业科学—蔬菜学] S642.2[农业科学—园艺学]