外源5-ALA与CaCl_(2)对露地栽培芍药生理及观赏品质的影响  

Effect of Exogenous 5-ALA and CaCl_(2)Treatment on Physiology and Ornamental Quality of Paeonia lactiflora in Open Field

作　　者：徐萌 张玉 李伟 XU Meng;ZHANG Yu;LI Wei(College of Landscape Architecture and Forestry,Qingdao Agricultural University,Qingdao,Shandong 266109,China;Rushan Forestry Bureau,Rushan,Shandong 265400,Chin)

机构地区：[1]青岛农业大学园林与林学院,山东青岛266109 [2]乳山市林业局,山东乳山265400

出　　处：《西北植物学报》2022年第6期1003-1011,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：青岛农业大学博士启动基金(663-1115006)。

摘　　要：该研究以露地栽培芍药品种‘大富贵’为材料,在叶面喷施100 mg·L^(-1)5-氨基乙酰丙酸(5-ALA)与4%CaCl_(2)后,4~7月份考察芍药叶片的质膜稳定性、抗氧化酶系统活性、叶绿素含量、光合气体交换参数以及观赏品质的变化,探究外源5-ALA和CaCl_(2)对高温胁迫下露地栽培芍药的抗逆生理生化机制,为缓解芍药夏季高温伤害提供技术依据。结果显示:(1)5-ALA与CaCl_(2)处理组的芍药植株花期、花径和花朵鲜重较对照组均显著增加。(2)与对照相比,5-ALA与CaCl_(2)处理后的芍药叶片能够维持较高的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性,较低的O^(-)·_(2)产生速率和MDA含量,以及较高的细胞叶绿素含量、净光合速率(P_(n))、蒸腾速率(T_(r))和气孔导度(G_(s))。(3)与叶面喷施CaCl_(2)相比,喷施5-ALA对露地栽培芍药‘大富贵’抗逆生理生化指标和观赏品质的改善效果更佳。研究表明,外源5-ALA与CaCl_(2)处理均可增加芍药的叶绿素含量和抗氧化能力,增强光合作用能力,降低膜脂过氧化水平,有效改善夏季露地栽培芍药‘大富贵’的观赏品质,并以100 mg·L-15-ALA的改善效果更佳。In order to explore the stress resistant physiological and biochemical mechanism of exogenous 5-aminolevulinic acid(5-ALA)and CaCl_(2)on Paeonia lactiflora cultivated in open field under high temperature stress,to provide technical basis for alleviating the high temperature injury of P.lactiflora in summer,we investigated the plasma membrane stability,antioxidant enzyme system,chlorophyll content,photosynthetic parameters and ornamental quality of P.lactiflora‘Dafugui’from April to July after spraying 100 mg/L 5-ALA and 4%CaCl_(2)on leaves in the study.The results revealed that:(1)the flowering period,flower diameter and fresh weight of P.lactiflora in 5-ALA and CaCl_(2)treatment groups were significantly higher than those in the control group;(2)Compared with the control,P.lactiflora treated with 5-ALA and CaCl_(2)could maintain higher superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD)activities,lower superoxide anion radical(O^(-)·_(2))production rate and MDA content,as well as higher cell chlorophyll content,net photosynthetic rate(P_(n)),transpiration rate(T_(r)),and stomatal conduct-ance(G_(s));(3)Compared with foliar spraying CaCl_(2),spraying 5-ALA had a better effect on the stress re-sistance physiological and biochemical indexes and ornamental quality of P.lactiflora cultivated in the open field.The results showed that exogenous 5-ALA and CaCl_(2)treatments could increase the chlorophyll content and antioxidant capacity of P.lactiflora,enhance the photosynthetic capacity,reduce the level of membrane lipid peroxidation,and effectively improve the ornamental quality of P.lactiflora‘Dafugui’cultivated in open field in summer,and the improvement effect of 100 mg·L^(-1)5-ALA was better.

关 键 词：芍药 5-氨基乙酰丙酸(5-ALA) CaCl_(2) 气体交换参数 叶绿素 抗氧化酶 

分 类 号：Q945.79[生物学—植物学] S682.12[农业科学—观赏园艺]