外源性TSG-6干预对支气管肺发育不良新生大鼠高氧肺损伤的保护作用及相关机制  

Protective effect and related mechanism of exogenous TSG-6 intervention on hyperoxia-induced lung injury in neonatal rats with bronchopulmonary dysplasia

作　　者：祝巍[1] 孙智勇[1] 王立君[2] 沈文生 王静竹[1] 高燕[1] 彭峰[1] 王书兰[1] 徐健[1] 方聪 韩明 孙秀红[1] ZHU Wei;SUN Zhi-yong;WANG Li-jun(Department of Neonatology,Jilin Provincial Maternal and Child Health Hospital,Changchun Jilin 130061,China;Department of Pediatric Respiratory Medicine,the First Hospital of Jilin University,Changchun Jilin 130061,China)

机构地区：[1]吉林省妇幼保健院新生儿科,吉林长春130061 [2]吉林大学第一医院小儿呼吸科,吉林长春130061

出　　处：《临床和实验医学杂志》2022年第13期1351-1356,共6页Journal of Clinical and Experimental Medicine

基　　金：吉林省卫生与健康及技术创新项目(编号:2020J125)。

摘　　要：目的探讨外源性肿瘤坏死因子刺激蛋白6(TSG-6)干预对支气管肺发育不良新生大鼠高氧肺损伤的保护作用,并分析其机制。方法将80只新生大鼠按照随机数字表法分为4组,分别为空气对照组、高氧肺损伤模型组、TSG-6预防组和TSG-6治疗组,每组各20只。空气对照组不建模,其他3组通过吸高氧建立高氧肺损伤新生大鼠模型。其中,高氧肺损伤模型组置于高氧箱内持续吸高氧14 d;TSG-6预防组在置于高氧箱前2 h实施气管内注入TSG-6剂量0.25μg/g TSG-6,然后进行高氧肺损伤吸氧;TSG-6治疗组进行高氧肺损伤吸氧,在吸高氧第5天开始在气管内注入剂量0.25μg/g TSG-6,直至14 d。分别于干预后7 d和14 d,苏木精-伊红染色观察各组大鼠肺组织病理学情况并进行放射状肺泡计数(RAC),酶联免疫吸附试验试剂盒检测大鼠肺组织中TSG-6、血管内皮生长因子(VEGF)和血清白细胞介素6(IL-6)水平,脂质过氧化丙二醛试剂盒检测血清丙二醛含量,总超氧化物歧化酶(SOD)活性试剂盒检测各血清SOD活性。结果干预7~14 d后,相较于高氧肺损伤组,TSG-6预防组和治疗组支气管上皮结构完整,上皮细胞形态结构正常、排列紧密;组织中可见肺泡壁轻度增厚,但TSG-6治疗组较预防组可见较多淋巴细胞与中性粒细胞浸润。干预后14 d,与高氧模型组相比,TSG-6预防组和TSG-6治疗组RAC、TSG-6、VEGF和SOD水平均显著增加,TSG-6预防组也显著高于TSG-6治疗组,差异均有统计学意义(P<0.05);与高氧模型组相比,TSG-6预防组和TSG-6治疗组IL-6、丙二醛水平均显著降低,TSG-6预防组也显著低于TSG-6治疗组,差异均有统计学意义(P<0.05)。与干预后7 d相比,干预后14 d,TSG-6预防组和TSG-6治疗组以上指标均显著改善,差异均有统计学意义(P<0.05)。结论TSG-6干预治疗可显著改善高氧所致肺损伤的病理学程度,对支气管上皮细胞形态结构具有保护作用,并可避免大鼠肺泡减�Objective To investigate the protective effect of exogenous tumor necrosis factor-stimulated protein 6(TSG-6)intervention on hyperoxia-induced lung injury in neonatal rats with bronchopulmonary dysplasia,and to analyze its mechanism.Methods Eighty neonatal rats were divided into 4 groups according to the random number table method.They were air control group,hyperoxia lung injury model group,TSG-6 prevention group and TSG-6 treatment group,20 rats in each group.The air control group was not modeled,and the other three groups established hyperoxia-induced lung injury neonatal rat models by inhaling hyperoxia.Among them,the hyperoxia lung injury model group was placed in a hyperoxia box and continuously inhaled hyperoxia for 14 days;the TSG-6 prevention group received intratracheal injection of TSG-6 at a dose of 0.25μg/g TSG-62 hours before being placed in the hyperoxia box,then,oxygen inhalation for hyperoxia lung injury was performed;in the TSG-6 treatment group,oxygen inhalation for hyperoxia lung injury was performed,and a dose of 0.25μg/g TSG-6 was injected into the trachea from the 5th day of hyperoxia inhalation until the 14th day.On 7 d and 14 d after the intervention,the histopathological conditions of the lung tissue of the rats in each group were observed by hematoxylin and eosin staining and the radial alveolar count(RAC)was performed,the levels of TSG-6,vascular endothelial growth factor(VEGF)in rat lung tissue and serum interleukin 6(IL-6)were detected by enzyme-linked immunosorbent assay kit,the content of malondialdehyde(MDA)was detected by the lipid peroxidation MDA kit,and the SOD activitywas detected by total SOD activity detection kit.Results After 7-14 days of intervention,compared with the hyperoxia lung injury group,the bronchial epithelial structure of the TSG-6 prevention group and the treatment group was complete,the epithelial cell morphology and structure were normal and closely arranged;the alveolar wall was slightly thickened in the tissue,however,more lymphocytes and neutrophils we

关 键 词：新生大鼠 外源性肿瘤坏死因子刺激蛋白6 肺发育不良 高氧肺损伤 机制 

分 类 号：R722.6[医药卫生—儿科]