Contrasting soil fungal communities in Mediterranean pine forests subjected to different wildfire frequencies  

作　　者：Erika Buscardo Susana Rodríguez-Echeverría Helena Freitas Paolo De Angelis João Santos Pereira Ludo A.H.Muller 

机构地区：[1]Centro de Ecologia Funcional(CEF),Departamento de Ciências da Vida,Universidade de Coimbra,Calçada Martim de Freitas,3000-456 Coimbra,Portugal [2]Department for Innovation in Biological,Agro-Food and Forest Systems(DIBAF),University of Tuscia,Via S.Camillo de Lellis snc,01100 Viterbo,Italy [3]Escritório Central do LBA,Instituto Nacional de Pesquisa da Amazônia(INPA),Av.AndréAraújo,2936,Campus II,Aleixo,69060-001 Manaus,Amazonas,Brazil [4]Departamento de Engenharia Florestal,Instituto Superior de Agronomia,Universidade Técnica de Lisboa,Tapada da Ajuda,1349-018 Lisbon,Portugal [5]Institut für Biologie—Botanik,Freie Universität Berlin,Altensteinstrasse 6,14195 Berlin,Germany

出　　处：《Fungal Diversity》2015年第1期85-99,共15页真菌多样性（英文）

基　　金：supported by the Portuguese Foundation for Science and Technology(FCT)through a Ph.D.grant for E.B.(SFRH/BD/21730/2005).

摘　　要：Mediterranean forest ecosystems are characterized by various vascular plant groups with their associated mycor-rhizae and free living soil fungi with various ecological functions.Fire plays a major role in Mediterranean ecosystem dynamics and impacts both above-and below-ground community structure and functioning.However,studies on the effects induced by altered disturbance regimes(associated with recent land use and climate extremes)on fire ecology and especially on its below-ground impacts are few.The objectives of this study were to evaluate the effects of different wildfire regimes on soil fungal community structure using two different molecular methods.We investigated the long-term effects of wildfire on soil fungal communities associated with Pinus pinaster forests in central Portugal,by comparing the results of denaturing gradient gel electrophoresis(DGGE)-based profiling with those obtained with 454 pyrosequencing.Four forest stands with differing fire history and fire return interval,and vegetation cover(mature forest,early successional stage of pine regeneration,and forest converted to scrubland)were sampled 6 years after the last fire event.The pyrosequencing-based approach indicated ca.eight-fold higher numbers of taxa than DGGE.However,fungal community fingerprinting data obtained for the different study stands with DGGE were congruent with those obtained with pyrosequencing.Both short(7.6 years)and long(24 years)fire return intervals(indicated by the presence of ericaceous shrubs in the understorey)induced a decrease in the abun-dance ratio between basidiomycetes and ascomycetes and appeared to reduce the frequency of ectomycorrhizal fungal species and saprophytes.Wildfire significantly reduced the frequency of late stage successional taxa(e.g.Atheliaceae and Cantharellales)and known or putative saprophytes belonging to the Clavulinaceae and the Archaeorhizomycetaceae.Conversely,early successional fungal species belonging to the Thelephoraceae were favoured by both fire return inter-vals,while the a

关 键 词：454 Pyrosequencing DGGE Wildfire frequency Soil fungal community Maritime pine 

分 类 号：S71[农业科学—林学]