两种质量浓度磷肥胁迫后柴胡各部位含皂苷量变化  

Changes of saponin content in different parts of Bupleurum chinense under two concentrations of phosphate fertilizer stress

作　　者：苗方[1] 孙佳琳[2] 李伟男[3] MIAO Fang;SUN Jia-lin;LI Wei-nan(Heze Medical College,Heze 274000,China;Heilongjiang Vocational College for Nationalities,Harbin 150020,China;Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China)

机构地区：[1]菏泽医学专科学校,山东菏泽274000 [2]黑龙江民族职业学院,哈尔滨150020 [3]黑龙江中医药大学,哈尔滨150040

出　　处：《哈尔滨商业大学学报（自然科学版）》2022年第4期392-396,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：山东省医药卫生科技发展计划项目,课题编号:2018WS499。

摘　　要：研究分别在高/低两种磷肥质量浓度胁迫后,柴胡植株各部位中含柴胡皂苷a、c、d量的变化规律.建立高效液相色谱法同时测定柴胡皂苷a、c、d的方法,并测定高/低两种质量浓度磷肥胁迫后,柴胡植株各部位中含三种皂苷成分的量.建立的同时测定含三种皂苷量的方法符合方法学要求.相比于对照组,低质量浓度磷肥胁迫后,柴胡植株各部位中含柴胡皂苷a的量会增加;高质量浓度磷肥胁迫后,主茎中含三种柴胡皂苷量均增加.高/低质量浓度磷肥分别胁迫后,会对柴胡各部位中含柴胡皂苷的量产生影响.To study the changes of saikosaponin a,c and d contents in different parts of Bupleurum chinense under high/low phosphate concentration stress.A method for simultaneous determination of saikosaponin a,c and d by high-performance liquid chromatography was established,and the contents of three saponins in various parts of saikosaponin plant after high/low concentration phosphate fertilizer stress were determined.The established method for simultaneous determination of three saponins met the requirements of methodology.Compared with the control group,the content of saikosaponin a in all parts of Bupleurum chinense plant will increase after low concentration phosphate fertilizer stress;after high concentration phosphate fertilizer stress,the contents of three saikosaponin in the main stem increased.The content of saikosaponin in different parts of Bupleurum chinense will be affected by high/low concentration phosphate fertilizer respectively.

关 键 词：柴胡皂苷A 柴胡皂苷c 柴胡皂苷D 含量测定 高效液相色谱法 磷胁迫 

分 类 号：R284.1[医药卫生—中药学]