人脐带血间充质干细胞移植对子宫内膜修复作用的临床研究  

Clinical study of human umbilical cord blood mesenchymal stem cell transplantation on endometrial repair

作　　者：王小红 林纯珍 王志君 葛爱娟 范晶晶 乐瑶 WANG Xiao-hong;LIN Chun-zhen;WANG Zhi-jun(Department of Gynaecology and Obstetrics,the PLA Joint Logistics Support Force 904 Hospital,Wuxi Jiangsu 214044,China)

机构地区：[1]解放军联勤保障部队第904医院妇产科,江苏无锡214044

出　　处：《临床和实验医学杂志》2022年第14期1534-1537,共4页Journal of Clinical and Experimental Medicine

基　　金：江苏大学临床医学科技发展基金项目(编号:JLY20160201);解放军联勤保障部队第904医院院级课题支持项目(编号:MS202111)。

摘　　要：目的探讨人脐带血间充质干细胞移植对子宫内膜修复作用的临床研究。方法前瞻性选取2020年9月至2021年10月入解放军联勤保障部队第904医院要求终止妊娠行经阴道型超声引导下全身静脉麻醉人工流产术的40例患者作为研究对象。随机数字表法分为对照组和间充质干细胞组,每组20例,对照组患者人工流产术后常规抗炎,予去氧孕烯炔雌醇片口服;间充质干细胞组在对照组的基础上,接受宫腔注射脐带血间充质干细胞治疗。比较两组患者子宫内膜厚度和子宫动脉血流情况,阴道出血天数和内膜呈三线征情况,下次月经复潮时间和复潮后经期时间,子宫内膜雌激素受体、孕激素受体、血管内皮生长因子水平,以及不良反应发生情况。结果术后第14天和第21天间充质干细胞组的子宫内膜厚度为(6.64±0.89)、(9.46±1.03)mm,明显优于对照组[(4.82±0.76)、(7.93±1.12)mm],差异均有统计学意义(P<0.05)。术后第14天和第21天间充质干细胞组的搏动指数和阻力指数分别为1.16±0.31、1.06±0.27和0.59±0.08、0.62±0.07,均明显低于对照组(2.19±0.57、1.98±0.29和0.93±0.12、0.87±0.11),差异均有统计学意义(P<0.05)。间充质干细胞组中出血≤7 d、内膜三线征患者占比65.00%、85.00%,显著高于对照组(40.00%、75.00%),差异均有统计学意义(P<0.05)。间充质干细胞组中复潮时间和复潮后经期时间为(34.61±5.09)、(5.19±1.27)d,明显短于对照组[(38.13±6.35)、(7.36±1.93)d],差异均有统计学意义(P<0.05)。间充质干细胞组子宫内膜组织中孕激素受体和血管内皮生长因子水平为8.39±0.26、8.15±0.16,均明显高于对照组(7.39±0.23、5.46±0.73),差异均有统计学意义(P<0.05)。两组患者的不良反应发生率差异无统计学意义(P>0.05)。结论人脐带血间充质干细胞移植是治疗子宫内膜修复的一种安全有效的方法,可有效提高子宫内膜厚度,改善子宫动脉血流情况Objective To investigate the clinical effect of human umbilical cord blood mesenchymal stem cell transplantation on endometrial repair.Methods A total of 40 patients who were admitted to the PLA Joint Logistics Support Force 904 Hospital from September 2020 to October 2021 for termination of pregnancy and general intravenous anesthesia induced abortion under transvaginal ultrasound guidance were selected as the research objects.The patients in the control group were randomly divided into control group and mesenchymal stem cell group,20 cases in each group.The patients in the control group were treated with marvelon after induced abortion,the mesenchymal stem cell group received intrauterine injection of umbilical cord blood mesenchymal stem cells on the basis of the control group.Endometrial thickness and uterine artery blood flow,vaginal bleeding days and endometrial three-line sign,time of next menstrual rehydration and post-rehydration menstrual period,endometrial estrogen receptors,progesterone receptors,vascular endothelial growth factor levels,and incidence of adverse reactions were compared.Results The endometrial thickness of the mesenchymal stem cell group on the 14th and 21st days after surgery were(6.64±0.89),(9.46±1.03)mm,which were significantly better than those of the control group[(4.82±0.76),(7.93±1.12)mm],the differences were statistically significant(P<0.05).The pulsatility index and resistance index of the mesenchymal stem cell group were 1.16±0.31,1.06±0.27 and 0.59±0.08,0.62±0.07 on the 14th and 21st days after surgery,which were significantly lower than those in the control group(2.19±0.57,1.98±0.29,and 0.93±0.12,0.87±0.11),the differences were statistically significant(P<0.05).The proportions of patients with bleeding≤7 days and endometrial three-line sign in the mesenchymal stem cell group were 40.00%,75.00%,which were significantly higher than those in the control group(65.00%and 85.00%),the differences were statistically significant(P<0.05).The rehydration time and menstru

关 键 词：人脐血间充质干细胞移植 子宫内膜修复 子宫动脉厚度 子宫内膜血流 

分 类 号：R711.74[医药卫生—妇产科学]