不同灭菌方法对檀香清咽片生药粉灭菌效果及主要成分含量的影响  

Effects of different sterilization methods on the sterilization effect and content of the major component in the raw powder of sandalwood throat-clearing tablets

作　　者：陈凤英 万丹[2] 邵国强[2] 李跃辉[2] 李鹏辉 扎西次仁 达瓦桑珠 CHEN Fengying;WAN Dan;SHAO Guoqiang;LI Yuehui;LI Penghui;ZHAXI Ciren;DAWA Sangzhu(Lixian Hospital of Traditional Chinese Medicine,Hunan,Lixian 415500,China;Hunan Academy of Chinese Medicine,Hunan,Changsha 410013,China;Shannan Tibetan Medical Hospital,Tibet,Shannan 856100,China)

机构地区：[1]湖南省澧县中医医院,湖南澧县415500 [2]湖南省中医药研究院,湖南长沙410013 [3]西藏自治区山南市藏医医院,西藏山南856100

出　　处：《中国医药科学》2022年第13期82-85,共4页China Medicine And Pharmacy

基　　金：西藏自治区科技计划项目(XZ201901-GA-05)。

摘　　要：目的 建立龙胆苦苷含量测定方法,比较不同灭菌方法对檀香清咽片生药粉灭菌效果及主要成分的影响,为檀香清咽片灭菌工艺的选择提供参考。方法 采用水蒸气蒸馏法测定檀香清咽片中挥发油的含量;采用高效液相色谱法测定檀香清咽片中主要成分龙胆苦苷的含量,以甲醇:0.1%磷酸溶液(20:80)为流动相,流速为1.0 ml/min,检测波长为270 nm;采用微生物计数法测定灭菌率;以灭菌率、挥发油和龙胆苦苷含量为考察指标,考察湿热灭菌、微波灭菌和乙醇灭菌方法对檀香清咽片生药粉的影响。结果 檀香清咽片生药粉采用75%乙醇灭菌时,微生物限度符合药典要求,且挥发油提取率及龙胆苦苷含量和灭菌前样品差异最小。结论 建立龙胆苦苷含量测定方法,该方法操作简便,为檀香清咽片质量控制研究提供理论依据;乙醇灭菌方法适用于檀香清咽片生药粉的灭菌,可为檀香清咽片的生产提供依据。Objective To establish a method for the determination of gentiopicroside content, to compare the effects of different sterilization methods on the sterilization effect and main component in the raw powder of sandalwood throat-clearing tablets, so as to provide a reference for the selection of sterilization process of sandalwood throat-clearing tablets. Methods The content of volatile oil in sandalwood throat-clearing tablets was determined by the steam distillation method. The content of gentiopicroside, the main component of sandalwood throat-clearing tablets, was determined by high performance liquid chromatography with methanol : 0.1% phosphoric acid solution(20:80) as the mobile phase, the flow rate set to 1.0 ml/min,and the detection wavelength set to 270 nm. The sterilization rate was determined by the microbial counting method. The effects of moist heat sterilization, microwave sterilization and ethanol sterilization methods on the raw powder of sandalwood throat-clearing tablets were investigated with the sterilization rate, volatile oil and gentiopicroside content as indexes. Results The microbial limits of the raw powder of sandalwood throatclearing tablets sterilized by 75% ethanol were in accordance with the pharmacopoeia requirements, and the extraction rate of volatile oil and gentiopicroside content was the least different from those of the sample before sterilization. Conclusion The established method for the determination of gentiopicroside content is simple,which can provide a theoretical basis for the quality control study of sandalwood throat-clearing tablets. The ethanol sterilization method is suitable for the sterilization of the raw powder of sandalwood throat-clearing tablets, which can be used as a basis for the production of sandalwood throat-clearing tablets.

关 键 词：檀香清咽片生药粉 湿热灭菌 微波灭菌 乙醇灭菌 龙胆苦苷 

分 类 号：R282.4[医药卫生—中药学]