CRISPR/Cas基因编辑系统在水稻耐盐碱研究中的应用  

Application of CRISPR/Cas Gene Editing System in Saline-Alkali Tolerance of Rice

作　　者：冷春旭[1,2,3] 闫平 徐振华[1,2,3] 孙中义 张书利[1,2,3] 武洪涛 吴立成[1,2,3] LENG Chun-xul;YAN Ping-;XU Zhen-hua;SUN Zhong-yi;ZHANG Shu-li;WU Hong-tao;WU Li-cheng(Biotechnology Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150023,China;Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang,Harbin 150023,China;Wuchang Experimental Station,Northeast Branch of National Center of Technology Innovation for Saline-Alkali Tolerant Rice,Harbin 150023,China)

机构地区：[1]黑龙江省农业科学院生物技术研究所,黑龙江哈尔滨150023 [2]黑龙江省作物与家畜分子育种重点实验室,黑龙江哈尔滨150023 [3]国家耐盐碱水稻技术创新中心东北中心五常试验站,黑龙江哈尔滨150023

出　　处：《黑龙江农业科学》2022年第8期52-56,共5页Heilongjiang Agricultural Sciences

基　　金：黑龙省生物育种科技重大专项(2020ZX16B01013);国家现代农业产业技术体系(CARS-01-54);黑龙江省农业科学院乡村振兴科技支撑示范项目(TGY-2021-02重点技术).

摘　　要：盐碱胁迫是限制水稻生长发育和阻碍农业生产的重要因素,是世界范围内造成农作物产量损失的主要原因之一.CRISPR/Cas基因编辑系统是一种高效简单的基因编辑技术,在植物非生物胁迫响应研究方面具有重要的应用价值.本文主要介绍了CRISPR/Cas基因编辑系统的发展过程、分类以及水稻对盐碱胁迫的响应机制,论述了近年来CRISPR/Cas技术在水稻耐盐碱品种开发中的应用,并对其应用前景进行了展望.Saline alkali stress has a significant impact on rice development and productivity and is one of the leading causes of crop yield losses in agricultural productivity worldwide.CRISPR/Cas gene editing system is an efficient and simple gene editing technology,which has important application value in abiotic stress of plants.In this paper,we introduced the development process,classification and function of CRISPR/Cas gene editing system,as well as the response mechanism of rice to saline-alkali stress and reviewed the application of the CRISPR/Cas technology in developing saline-alkali tolerant rice varieties in recent years,and prospected its application prospect.

关 键 词：基因编辑 CRISPR/Cas系统 水稻 耐盐碱 

分 类 号：S511[农业科学—作物学]