Diversity of foliar endophytes in wind-fallen Picea abies trees  

作　　者：Ondřej Koukol Miroslav Kolařík Zuzana Kolářová Petr Baldrian 

机构地区：[1]Department of Botany,Faculty of Science,Charles University in Prague,Benátská2,12801 Prague 2,Czech Republic [2]Laboratory of Genetics,Physiology and Bioengineering of Fungi,Institute of Microbiology of the ASCR,v.v.i.,Vídeňská1083,14220 Prague 4,Czech Republic [3]Laboratory of Environmental Microbiology,Institute of Microbiology of the ASCR,v.v.i.,Vídeňská1083,14220 Prague 4,Czech Republic

出　　处：《Fungal Diversity》2012年第3期69-77,共9页真菌多样性（英文）

基　　金：supported by the Grant Agency of the Czech Republic(project Nr.526/08/0751);the Ministry of Education,Youth and Sports of the Czech Republic(project No.MSM0021620828);the Institutional Research Concept of the Institute of Microbiology of the ASCR,v.v.i.(AV0Z50200510).

摘　　要：The unmanaged Norway spruce montane forests in the Bohemian Forest National Park(Czech Republic)suffered from repeated large-scale bark beetle outbreaks in last decade.In this study,the diversity of culturable foliar endophyte microfungi in needles originating from eleven recently wind-fallen trees in this area was surveyed.Our aims were to describe their diversity and to determine the relative host and organ specificities of isolated endophyte species to estimate the species pool and abundance of foliar endophytes before the forest degradation.Microfungi were isolated from surface-sterilized needles,and the outgrowing strains were identified based on morphological and molecular characteristics(analyses of ITS1,ITS2 and partial 28S rDNA).Fungal communities in the needles were diverse,with ascomycetes(mostly anamorphs of Helotiales)dominating basidiomycetes.The most frequent species(Phacidiopycnis sp.,Cistella acuum,Sirococcus sp.and two species of Chalara)did not correspond with those recorded in previous studies of Picea spp.For example,the widely distributed Lophodermium piceae was rarely recorded in this study.This pattern may be caused by different methods of sterilization and cultivation or by physiological characteristics of the needles,or it may reflect the species distribution in the studied area.Members of the Helotiales,along with sequences from GenBank,showed substantial overlap in host affinities,most prominently between Pinus and Picea,and also among species from distant plant lineages.

关 键 词：MORPHOSPECIES ITS rDNA Culturable microfungi Phacidiopycnis Host affinity 

分 类 号：Q94[生物学—植物学]