不同砧木对赤霞珠葡萄种子内源激素和多胺含量的影响  

Effects of different rootstocks on contents of endogenous hormones and polyamines in Cabernet Sauvignon grape seeds

作　　者：赵书成 孙军利[1] 赵宝龙[1] 潘立忠[1] 章智钧 朱春梅 ZHAO Shucheng;SUN Junli;ZHAO Baolong;PAN Lizhong;ZHANG Zhijun;ZHU Chunmei(Agriculture College of Shihezi University/Key Laboratory of Special Fruits&Vegetables Cultivation Physiology and Germplasm Resources Utilization/The Key Laboratory of Oasis Eco-agriculture,Xingjiang production and Construction Corps,Shihezi 832003,Xinjiang,China)

机构地区：[1]石河子大学农学院·新疆生产建设兵团特色果蔬栽培生理与种质资源利用重点实验室·新疆生产建设兵团绿洲生态农业省部共建国家重点实验室,新疆石河子832003

出　　处：《果树学报》2022年第8期1422-1431,共10页Journal of Fruit Science

基　　金：国家自然科学基金项目(32060648,332060647)。

摘　　要：【目的】通过比较不同砧木嫁接的赤霞珠葡萄种子中相关激素和多胺含量变化趋势,为砧穗互作对果实激素、多胺代谢影响的研究提供理论基础。【方法】以赤霞珠(Cabernet Sauvignon,CS)自根苗为对照,以嫁接在5BB、SO4、140R、3309M、河岸葡萄砧木上的赤霞珠葡萄为试验材料,测定赤霞珠葡萄果实从转色初期至成熟期可滴定酸含量和还原糖含量,以及种子中赤霉素(gibberellins A3,GA3)、生长素(indole acetic acid,IAA)、脱落酸(abscisic acid,ABA)、腐胺(putrescine,Put)、亚精胺(spermidine,Spd)、精胺(spermine,Spm)的含量。【结果】供试葡萄砧木品种均可以降低赤霞珠葡萄果实可滴定酸含量,提高还原糖含量;随着果实发育,不同砧穗组合种子中GA3含量总体呈“M”型变化趋势,Spd和IAA含量持续降低,ABA含量出现先降低后升高的变化趋势,而Put、Spm含量呈先上升后降低的变化趋势;在果实转色期各砧穗组合不同程度地提高了赤霞珠葡萄种子中Put和Spm含量,降低了IAA、ABA、Spd含量;而在果实成熟期各砧木降低了IAA和Spm含量,提高了GA3、ABA、Put、Spd含量。【结论】与自根苗相比,嫁接明显改变了赤霞珠葡萄种子中激素和多胺的代谢水平,从而影响葡萄果实成熟。【Objective】Xinjiang is the earliest region to grow grape and also a major province for wine grape cultivation and brewing in China.However,Xinjiang's diverse climate,drought,salinity,cold and other ecological conditions restrict the further development of wine grape industry,resulting in a significant reduction in grape yield and quality.The grape grafting can adapt to different soil types and growth environments of biological(like soil pests)and non-biological(such as salinity,moisture or temperature)stresses.Plant hormones and polyamines are compounds,which derive through many important metabolic pathways in plants.Not only can they regulate plant growth and metabolism,but are also be closely related to stress resistance.This study compared the trend of changes in the content of related hormones and polyamines in seeds of Cabernet Sauvignon grapevine grafted on different rootstocks.It can provide a theoretical basis for the research on the effect of scion-rootstock interaction on fruit hormone and polyamine metabolism.【Methods】Five resistant rootstocks(5BB,V.riparia,SO4,140R and 3309M)were collected all from Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences.The scion was Cabernet Sauvignon 169(CS),which was planted in the grape test garden in 2014.The rootstock-scion combinations included CS/5BB,CS/V.riparia,CS/SO4,CS/140R and CS/3309M.The experiment started on July 28,2020(berry veraison period),until the end of sampling on September 8,2020(fruit ripening period).Sampling was completed once every 10 days,totaling for 5 sampling times.Random samples were taken during each sampling and brought back to the laboratory in time.The pulp and seeds were accurately separated and quickly frozen in liquid nitrogen,and 0.5 g of each sample was weighed,which was repeated for 3 times.Then the samples were placed in a refrigerator at-80°C.Determination of titratable acid and reducing sugar contents in berries and the contents of gibberellins A3(GA3),indole acetic acid(IAA)and abscisic acid(

关 键 词：葡萄 砧木 抗性 内源激素 多胺 

分 类 号：S663.1[农业科学—果树学]