Front line defenders of the ecological niche!Screening the structural diversity of peptaibiotics from saprotrophic and fungicolous Trichoderma/Hypocrea species  

作　　者：Christian R.Rohrich Walter M.Jaklitsch Hermann Voglmayr Anita Iversen Andreas Vilcinskas Kristian Fog Nielsen Ulf Thrane Hans von Dohren Hans Bruckner Thomas Degenkolb 

机构地区：[1]Bioresources Project Group,Fraunhofer Institute for Molecular Biology and Applied Ecology(IME),Winchesterstrasse 2,35394 Giessen,Germany [2]Department of Systematic and Evolutionary Botany,Faculty Centre of Biodiversity,University of Vienna,Rennweg 14,1030 Vienna,Austria [3]Department of Systems Biology,Technical University of Denmark,Soltofts Plads,Building 221,2800 Kgs.Lyngby,Denmark [4]Interdisciplinary Research Centre for BioSystems,Land Use and Nutrition(IFZ),Department of Applied Entomology,Institute of Phytopathology and Applied Zoology(IPAZ),University of Giessen,Heinrich-Buff-Ring 26-32,35392 Giessen,Germany [5]Biochemistry and Molecular Biology OE 2,Institute of Chemistry,Technical University of Berlin,Franklinstrasse 29,10587 Berlin,Germany [6]Interdisciplinary Research Centre for BioSystems,Land Use and Nutrition(IFZ),Department of Food Sciences,Institute ofNutritional Science,University of Giessen,Heinrich-Buff-Ring 26-32,35392 Giessen,Germany [7]AB SCIEXGermany GmbH,Landwehrstrasse 54,64293 Darmstadt,Germany [8]Danish Emergency Management Agency,Universitetsparken 2,2100 Copenhagen,Denmark

出　　处：《Fungal Diversity》2014年第6期117-146,共30页真菌多样性（英文）

基　　金：supported by the Hessian Ministry for Science and Art by a grant from the LOEWE-Schwerpunkt‘Insect Biotechnology’to Andreas Vilcinskas;the grant from the Danish Research Council(FI 2136-08-0023)for the maXis QTOF system;MYCORED(EC KBBE-2007-222690-2)for supporting Anita Iversen;the support by the Austrian Science Fund(project P22081-B17).

摘　　要：Approximately 950 individual sequences of nonribosomally biosynthesised peptides are produced by the genus Trichoderma/Hypocreathat belong to a perpetually growing class of mostly linear antibiotic oligopeptides,which are rich in the non-proteinogenic α-aminoisobutyric acid(Aib).Thus,they are comprehensively named peptaibiotics.Notably,peptaibiotics represent ca.80% of the total inventory of secondarymetabolites currently known from Trichoderma/Hypocrea.Their unique membrane-modifying bioactivity results from amphipathicity and helicity,thus making them ideal candidates in assisting both colonisation and defence of the natural habitats by their fungal producers.Despite this,reports on the in vivo-detection of peptaibiotics have scarcely been published in the past.In order to evaluate the significance of peptaibiotic production for a broader range of potential producers,we screened nine specimens belonging to seven hitherto uninvestigated fungicolous or saprotrophic Trichoderma/Hypocrea species by liquid chromatography coupled to electrospray high resolution mass spectrometry.Sequences of peptaibiotics found were independently confirmed by analysing the peptaibiome of pure agar cultures obtained by single-ascospore isolation from the specimens.Of the nine species examined,five were screened positive for peptaibiotics.A total of 78 peptaibiotics were sequenced,56(=72%)of which are new.Notably,dihydroxyphenylalaninol and O-prenylated tyrosinol,two C-terminal residues,which have not been reported for peptaibiotics before,were found as well as new and recurrent sequences carrying the recently described tyrosinol residue at their C-terminus.The majority of peptaibiotics sequenced are 18-or 19-residue peptaibols.Structural homologies with‘classical representatives’of subfamily 1(SF1)-peptaibiotics argue for the formation of transmembrane ion channels,which are prone to facilitate the producer capture and defence of its substratum.

关 键 词：HPLC/QTOF-ESI-HRMS Metabolite profiling Peptaibiotics PEPTAIBOLS Aib peptides TRICHODERMA HYPOCREA 

分 类 号：F42[经济管理—产业经济]