A new endophytic insect-associated Daldinia species, recognised from a comparison of secondary metabolite profiles and molecular phylogeny  

作　　者：Sylvie Pažoutová Stefanie Follert Jens Bitzer Matthias Keck Frank Surup PetrŠrůtka Jaroslav Holuša Marc Stadler 

机构地区：[1]Institute of Microbiology of the ASCR,v.v.i.,Vídeňská1083,14220 Prague 4,Czech Republic [2]Department Microbial Drugs,Helmholtz-Zentrum für Infektionsforschung GmbH,Inhoffenstrasse 7,38124 Braunschweig,Germany [3]IMD Natural Solutions GmbH,Otto-Hahn-Strasse 15,44227 Dortmund,Germany [4]Faculty of Forestry and Wood Sciences,Czech University of Life Sciences,Kamýcká129,16521 Prague 6,Czech Republic

出　　处：《Fungal Diversity》2013年第3期107-123,共17页真菌多样性（英文）

基　　金：support from COST Action FA1103“Endophytes in Biotechnologiy and Agriculture”.

摘　　要：Xylariaceous endophytes are commonly encountered in plants,and it has recently been found out that insect vectors play a major role in their life cycle,as well as in the apparent host-specificity of the fungal stromata.In this study,a new insect-associated,endophytic anamorph species of Daldinia is recognised from a comparison of (a)morphological and ecological data;(b)molecular phylogenetic data based on rDNA,alpha-actin and beta-tubulin genes;and (c)secondary metabolite profiles based on high performance liquid chromatography/diode array/mass spectrometry(HPLC-DAD/MS)and gas chromatography/mass spectrometry(GC-MS)in comparison with authentic strains of Daldinia and other Xylariaceae.The new species,Daldinia hawksworthii,is a symbiont of the willow woodwasp,Xiphydria prolongata,which is in turn preferentially associated with Salix.A comparison of HPLCDAD/MS profiles from standardised cultures revealed the presence of a specific compound,which was not encountered in any of the ca.250 Daldinia strains studied for comparison.This compound was isolated after fermentation of the fungus in 10 l scale by preparative chromatography.Structure elucidation by NMR spectroscopy revealed a novel pyrone,for which the name dalsymbiopyrone is proposed.The compound showed weak antimicrobial and cytotoxic effects and might be a signal molecule involved in the trilateral ecological interaction between the host plant,the insect,and its fungal symbiont.

关 键 词：FUNGI ENDOPHYTES Evolution XYLARIALES Bioactive metabolites Natural products Alpha-pyrones 

分 类 号：O62[理学—有机化学]