Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli  

作　　者：Xiaobo Ruan Sheng Zhang Wei Song Jia Liu Xiulai Chen Liming Liu Jing Wu 

机构地区：[1]School of Pharmaceutical Science,Jiangnan University,Wuxi 214122,China [2]State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China [3]Tianrui Chemical Co.,Ltd,Department of Chemistry,Quzhou 324400,China

出　　处：《Chinese Journal of Chemical Engineering》2022年第7期18-30,共13页中国化学工程学报（英文版）

基　　金：financially supported by the Fundamental Research Funds for the Central Universities (JUSRP21915);National Natural Science Foundation of China (22008089, 21878126);Provincial Natural Science Foundation of Jiangsu Province(BK20200622);the key technologies Research&Development Program of Jiangsu Province (BE2018623);the National First-Class Discipline Program of Light Industry Technology and Engineering(LITE2018-20)

摘　　要：For the efficient conversion of L-tyrosine(L-Tyr)to tyrosol,which is an aromatic compound widely used in the pharmaceutical and chemical industries,a novel four-enzyme cascade pathway based on the Ehrlich pathway of Saccharomyces cerevisiae was designed and reconstructed in Escherichia coli.Then,the expression levels of the relevant enzymes were coordinated using a modular approach and gene duplication after the identification of the pyruvate decarboxylase from Candida tropicalis(CtPDC)as the rate-limiting enzymatic step.In situ product removal(ISPR)strategy with XAD4 resins was explored to avoid product inhibition and further improve tyrosol yield.As a result,the titer and conversion rate of tyrosol obtained were 35.7 g·L^(-1) and 93.6%,respectively,in a 3-L bioreactor.Results presented here provide a potential enzymatic process for industrial production of tyrosol from cheap amino acids.

关 键 词：TYROSOL L-TYROSINE Ehrlich pathway Enzyme cascade In situ product removal 

分 类 号：TQ243.4[化学工程—有机化工]