人诱导多能干细胞及其在血管相关疾病模型中的应用  

Human induced pluripotent stem cell and its applications in vascular disease modeling

作　　者：胡敏洁 王思贤 王永煜 Hu Minjie;Wang Sixian;Wang Yongyu(College of Basic Medical Sciences,Wenzhou Medical University,Wenzhou 325035,China)

机构地区：[1]浙江省温州医科大学基础医学院,温州325035

出　　处：《中华细胞与干细胞杂志（电子版）》2022年第3期167-175,共9页Chinese Journal of Cell and Stem Cell（Electronic Edition）

基　　金：国家自然科学基金(82070487,81670454);浙江省自然科学基金(LY21C120003)。

摘　　要：诱导多能干细胞(iPSCs)是由体细胞重编程获得的一种与胚胎干细胞类似能够无限自我更新和分化成体内各种细胞类型的多能干细胞。iPSCs现已广泛应用于人类疾病的发病机制研究、治疗药物研发及再生医学等领域。血管疾病具有较高的发病率和死亡率,其发病机制常与血管平滑肌细胞(VSMCs)和内皮细胞(ECs)功能障碍有关。建立血管疾病特异性iPSCs并将其定向分化为VSMCs和ECs等,可作为研究血管疾病发病机制或进行药物筛选的重要体外细胞模型。此外,3D培养和其他技术的发展应用还可从3D水平模拟体内环境将iPSCs应用于疾病模型构建和发病机制研究。本综述旨在介绍血管疾病特异性iPSCs的获取、iPSCs定向诱导分化为SMCs和ECs的方法及其在各种血管疾病发病机制研究中的应用与挑战。Induced pluripotent stem cells(iPSCs)are reprogrammed from somatic cells,which is similar to embryonic stem cells with the capable of infinite self-renewal and differentiation into various types of cells.The iPSCs have been widely used in the pathogenesis of human disease modeling,drug development and regenerative medicine.Vascular diseases with high morbidity and mortality are often associated with the dysfunction of vascular smooth muscle cells(VSMCs)and endothelial cells(ECs).Establishment of patient-specific iPSCs and the derived-SMCs or ECs can provide an important cellular model for studying the pathogenesis of vascular diseases,as well as drug screening.In addition,the development and application of 3-Dimensions(3D)culture and other technologies can also simulate to generate the 3D constructs,such as organoid,organ on chip,and disease modeling.The purpose of this review is to introduce the generation of iPSCs,the differentiation methods of SMCs and ECs from iPSCs,as well as their applications and challenges in the pathogenesis of the various vascular diseases.

关 键 词：诱导多能干细胞 重编程 血管平滑肌细胞 内皮细胞 分化 3D模型 疾病模型 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]