乙肝后肝硬化患者Child- Pugh分级与细胞免疫功能相关性分析  

An Analysis of the Relationship Between Child- Pugh Score and Cellular Immune Function in Patients with Hepatitis B Cirrhosis

作　　者：李欣 赵培培 王富兵 丁江涛 范辉[1] 魏怡然 LI Xin;ZHAO Peipei;WANG Fubing;DING Jiangtao;FAN Hui;WEI Yiran(Department of Gastroenteritis,The Second People’s Hospital of Nantong City,Nantong 226002,China;Deparment of Geriatrics,The Second People’s Hospital of Nantong City,Natong 226002,China;Shanghai Jiaotong University,Shanghai 200025,China)

机构地区：[1]南通市第二人民医院消化内科,江苏南通226002 [2]南通市第二人民医院老年医学科,江苏南通226002 [3]上海交通大学,上海200025

出　　处：《标记免疫分析与临床》2022年第6期932-934,957,共4页Labeled Immunoassays and Clinical Medicine

基　　金：国家自然科学基金青年基金项目(编号:82000532)。

摘　　要：目的分析乙肝后肝硬化患者不同Child-Pugh评分级别与外周血T细胞亚群和NK细胞百分比变化的相关性。方法收集乙肝后肝硬化患者84例,Child-Pugh评分A级27例、B级29例、C级28例,对照组25例,采用流式细胞术测定肝硬化患者和对照组外周血T细胞亚群(CD3^(+)T、CD4^(+)T、CD8^(+)T)、NK细胞百分比,计算CD4^(+)/CD8^(+)T比率。结果和对照组相比,乙肝后肝硬化患者外周血淋巴细胞CD3^(+)T、CD4^(+)T、NK细胞百分比和CD4^(+)/CD8^(+)T比率降低(P<0.05);Child-Pugh A级CD3^(+)T、CD4^(+)T、NK细胞百分比和CD4^(+)T/CD8^(+)T比率高于B级,差异有统计学意义(P<0.05)。A、B级间CD8^(+)T淋巴细胞百分比差异无统计学意义(P>0.05)。Child-Pugh C级与A、B级比较,C级者CD3^(+)T、CD4^(+)T、NK细胞百分比和CD4^(+)T/CD8^(+)T比率均降低,组间差异有统计学意义(P<0.05)。结论在乙肝后肝硬化进展过程中,Child-Pugh评分C级患者外周血中CD3^(+)T、CD4^(+)T、NK细胞百分比和CD4^(+)T/CD8^(+)T比率均明显下降,提示Child-Pugh评分C级患者细胞免疫功能加速减退。Objective To analyze variations of T lymphocyte subtype,natural killer cells and the ratio of CD4^(+)T/CD8^(+)T in patients with hepatitis B cirrhosis of different Child-Pugh classifications.Methods The clinical data and laboratory characteristics of 84 patients with hepatitis B cirrhosis of different Child-Pugh classification were collected in the Second People’s Hospital of Nantong city.According to the results of Child-Pugh classification,27 cases were classified into grade A group,29 cases into grade B group,28 cases into grade C group,and 25 cases volunteers were enrolled into the control group.The peripheral blood samples of all the patients were collected to determine CD3^(+)T,CD4^(+)T,CD8^(+)T,and NK cells.And the ratio of CD4^(+)T/CD8^(+)T was calculated at the same time.Results Compared with the control group,percentages of CD3^(+)T,CD4^(+)T lymphocytes,NK cells and the ratio of CD4^(+)T/CD8^(+)T of all patients with liver cirrhosis after hepatitis B infection were declined,and all differences showed statistics significance(P<0.05).Percentages of CD3^(+)lymphocytes,CD4^(+)lymphocytes,NK cells and the ratio of CD4^(+)T/CD8^(+)T in grade A group were higher than that in grade B group(P<0.05).There was no significant difference in the percentage of the CD8^(+)T lymphocytes between A and B groups(P>0.05).Compared with grade A&B group,CD3^(+)lymphocytes,CD4^(+)lymphocytes,NK cells and the ratio of CD4^(+)T/CD8^(+)T in grade C group were declined,and the difference was of statistics significance(P<0.05).Conclusion With the deterioration of hepatitis B cirrhosis,percentages of CD3^(+)T,CD4^(+)lymphocytes,NK cells and the ratio of CD4^(+)/CD8^(+)of patients in grade C group are greatly decreased.In Child-Pugh grade C group,patients’cellular immune function are decreased rapidly.

关 键 词：乙肝后肝硬化 T细胞 NK细胞 CHILD-PUGH评分 

分 类 号：R378[医药卫生—病原生物学]