糖尿病大鼠牙槽骨中核因子-κB受体活化因子配体和骨保护素mRNA表达与骨密度变化之间的关系  

The Relationship Between mRNA Expression of Receptor Activator of Nuclear Factor- Kappa B Ligand and Osteoprotegerin in Alveolar Bone of Diabetic Rats and Changes in Bone Mineral Density

作　　者：李光武[1] 郑旭[1] 韩经定 LI Guangwu;ZHENG Xu;HAN Jingding(Department of Stomatology,the First Affiliated Hospital of Hainan Medical College,Haikou 570203,China)

机构地区：[1]海南医学院第一附属医院口腔科,海南海口570203

出　　处：《标记免疫分析与临床》2022年第6期981-986,1031,共7页Labeled Immunoassays and Clinical Medicine

基　　金：海南省教育厅高校科研重点项目(编号:Hnky2020ZD-19)。

摘　　要：目的探讨糖尿病大鼠牙槽骨中核因子-κB受体活化因子配体(RANKL)和骨保护素(OPG)mRNA表达与骨密度变化之间的关系。方法72只雄性大鼠随机选择12只正常饮食,其他60只制备糖尿病模型(DM组),将大鼠随机分为模型组、利拉鲁肽组与利拉鲁肽+吡咯烷二硫氨基甲酸(pyrrolidine dithiocarbamate,PDTC)组。造模期间监测大鼠体重,造模4周后测定随机血糖、空腹血糖;测定牙槽骨、全身骨密度水平,采用比色法测定抗酒石酸酸性磷酸酶-5b(TRACP-5b)、钙离子(Ca^(2+))、碱性磷酸酶(ALP)等骨代谢指标水平,采用反转录聚合酶链式反应测定牙槽骨RANKL、OPG mRNA表达水平;并分析牙槽骨RANKL、OPG mRNA表达水平与骨密度、骨代谢指标相关性。结果造模4周后,DM组大鼠体重、牙槽骨密度、全身骨密度、ALP下降,随机血糖、空腹血糖、TRACP-5b、Ca^(2+)、RANKL mRNA、OPG mRNA上升;利拉鲁肽组大鼠体重、牙槽骨密度、全身骨密度、ALP上升,随机血糖、空腹血糖、TRACP-5b、Ca^(2+)、RANKL mRNA、OPG mRNA下降;利拉鲁肽组大鼠加用PDTC处理后可以有效逆转应用利拉鲁肽处理DM大鼠效果。Pearson相关系数分析显示RANKL、OPG mRNA与牙槽骨密度、全身骨密度、ALP负相关(P<0.05),与TRACP-5b、Ca^(2+)正相关(P<0.05)。结论糖尿病造成大鼠骨密度降低,抗糖尿病处理可以有效抑制大鼠牙槽骨RANKL、OPG mRNA水平,调节骨代谢失衡,最终影响骨密度。Objective The purpose of this study was to investigate the relationship between mRNA expression of receptor activator of nuclear factor-kappa B ligand(RANKL)and osteoprotegerin(OPG)in alveolar bone of diabetic rats and changes in bone mineral density.Methods 12 of the 72 male rats selected were given normal diets,while the other 60 rats were used to prepare diabetes mellitus model(DM group).These rats were randomly divided into the model group,liraglutide group and liraglutide+pyrrolidine dithiocarbamate(PDTC)group.Weight of studied rats were monitored during modeling stage.Random blood glucose and fasting blood glucose were measured at 4 weeks after modeling.Alveolar bone and whole-body bone density were measured.Levels of bone metabolism indicators such as tartrate-resistant acid phosphatase 5b(TRACP-5b),calcium ion(Ca^(2+))and alkaline phosphatase(ALP)were determined by colorimetry.Reverse transcription polymerase chain reaction assay was performed to determine mRNA expression levels of RANKL and OPG in alveolar bone.The correlation between mRNA expression levels of RANKL and OPG in alveolar bone and bone mineral density,bone metabolism indicators was analyzed.Results 4 weeks after modeling,weight,alveolar bone density,whole-body bone density,and ALP level of the DM group decreased,while random blood glucose,fasting blood glucose,TRACP-5b,Ca^(2+),RANKL mRNA,and OPG mRNA increased.Weight,alveolar bone density,whole-body bone density,and ALP level of the liraglutide group increased,while random blood glucose,fasting blood glucose,TRACP-5b,Ca^(2+),RANKL mRNA,and OPG mRNA decreased.The additional treatment with PDTC for rats in the liraglutide group could effectively reverse the effect on rats with DM treated with liraglutide.Pearson correlation coefficient analysis showed that RANKL and OPG mRNAs were negatively correlated with alveolar bone density,whole-body bone density and ALP(P<0.05),while positively correlated with TRACP-5b and Ca^(2+)(P<0.05).Conclusion DM causes a decrease in bone mineral density of rats.

关 键 词：糖尿病 牙槽骨 核因子-ΚB受体活化因子配体 骨保护素 骨密度 

分 类 号：R587.1[医药卫生—内分泌]