难治性肺炎支原体肺炎患儿血清长链非编码RNA肺腺癌转移相关转录因子1和烟酰胺核苷酸反义转氢酶RNA1检测的临床意义  

Clinical Significance of Serumlong Non-coding RNA Metastasis-associated Lung Adenocarcinoma Transcript 1,Nicotinamide Nucleotide Transhydrogenase-antisense RNA1 in Children with Refractory Mycoplasma Pneumoniae Pneumonia

作　　者：冯华 薛洪刚 徐玉秀 FENG Hua;XUE Hong-gang;XU Yu-xiu(Department of Pediatrics,Fuxin Mine General Hospital of Liaoning Health Industry Group,Liaoning Fuxin 123000,China;Department of Respiratory Medicine,Fuxin Mine General Hospital of Liaoning Health Industry Group,Liaoning Fuxin 123000,China;Department of Clinical Laboratory,Fuxin Mine General Hospital of Liaoning Health Industry Group,Liaoning Fuxin 123000,China)

机构地区：[1]辽宁省健康产业集团阜新矿总医院儿科,辽宁阜新123000 [2]辽宁省健康产业集团阜新矿总医院呼吸内科,辽宁阜新123000 [3]辽宁省健康产业集团阜新矿总医院检验科,辽宁阜新123000

出　　处：《现代检验医学杂志》2022年第4期7-12,164,共7页Journal of Modern Laboratory Medicine

基　　金：2019年辽宁省自然科学基金指导计划(2019034017)。

摘　　要：目的探讨难治性肺炎支原体肺炎(refractory Mycoplasma pneumoniae pneumonia,RMPP)患儿血清长链非编码核糖核酸(long non-coding ribonucleic acid,LncRNA)肺腺癌转移相关转录因子1(metastasis-associated lung adenocarcinoma transcript 1,MALAT1)、烟酰胺核苷酸反义转氢酶RNA1(nicotinamide nucleotide transhydrogenase-antisense RNA1,NNT-AS1)检测的临床意义。方法选择2018年1月1日~2021年1月1日辽宁省健康产业集团阜新矿总医院收治的200例肺炎支原体肺炎患儿,其中43例为RMPP(RMPP组),157例为普通肺炎支原体肺炎(普通肺炎组),另选择100例健康儿童为对照组。检测血清LncRNA MALAT1和LncRNA NNT-AS1表达水平以及炎性因子[C反应蛋白(C-reactive protein,CRP)、降钙素原(procalcitonin,PCT)、白细胞介素-8(interleukin-8,IL-8)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)]水平。分析血清LncRNA MALAT1和LncRNA NNT-AS1表达水平与炎性因子水平的相关性。收集临床资料,分析影响RMPP发病的影响因素,比较不同疗效RMPP患儿血清LncRNA MALAT1和LncRNA NNT-AS1表达水平的差异。结果RMPP组、普通肺炎组和对照组血清LncRNA MALAT1(3.26±0.85,1.32±0.41和1.05±0.37)和LncRNA NNT-AS1(3.38±0.92,1.41±0.40和1.06±0.39)水平比较差异均有统计学意义(F=335.693,335.828,均P<0.05),血清CRP(45.24±2.01 mg/L,19.02±3.27 mg/L和3.26±0.77 mg/L),PCT(1.58±0.52μg/L,0.82±0.16μg/L和0.31±0.09μg/L),IL-8(42.77±8.84μg/L,26.35±5.91μg/L和13.02±3.79μg/L)和TNF-α(9.22±2.61μg/L,5.02±1.49μg/L和3.32±0.96μg/L)水平比较差异均有统计学意义(F=4207.164,457.187,410.300,214.875,均P<0.05)。Pearson相关性分析结果显示血清LncRNA MALAT1,LncRNA NNT-AS1表达水平均与CRP,PCT,IL-8,TNF-α水平呈正相关(r=0.715~0.922,均P<0.05)。Logistic逐步回归分析结果显示肺大片实变影[OR(95%CI)=2.212(1.396~3.506)]、CRP高表达[OR(95%CI)=2.111(1.313~3.392)]、LncRNA MALAT1高表达[OR(95%CI)=1.826(1.189~2.805)]、LncRNA NNT-AS1高表达[ORObjective To investigate the clinical significance of detecting serum long non-coding ribonucleic acid(LncRNA)metastasis-associated lung adenocarcinoma transcript 1(MALAT1)and nicotinamide nucleotide transhydrogenase-antisense RNA1(NNT-AS1)in children with refractory Mycoplasma pneumoniae pneumonia(RMPP).Methods A total of 200 children with Mycoplasma pneumoniae pneumonia who were admitted to Fuxin Mine General Hospital of Liaoning Health Industry Group from January 1,2018 to January 1,2021 were selected.Among them,43 cases were RMPP(RMPP group),157 cases were common Mycoplasma pneumoniae pneumonia(common pneumonia group),another 100 healthy children were selected as the control group.The expression levels of serum LncRNA MALAT1 and LncRNA NNT-AS1 and the levels of inflammatory factors[C-reactive protein(CRP),procalcitonin(PCT),interleukin-8(IL-8),tumor necrosis factor-α(TNF-α)]were detected.The correlation between the expression levels of serum LncRNA MALAT1 and LncRNA NNT-AS1 and the levels of inflammatory factors were analyzed.The clinical data were collected,the influencing factors of the incidence of RMPP were analyzed.The expression levels of serum LncRNA MALAT1 and LncRNA NT-AS1 in children with RMPP with different therapeutic effects were compared.Results There were significant differences in the levels of serum LncRNA MALAT1(3.26±0.85,1.32±0.41 and 1.05±0.37),LncRNA NNT-AS1(3.38±0.92,1.41±0.40 and 1.06±0.39)in RMPP group,common pneumonia group and control group(F=335.693,335.828,all P<0.05),and there were significant differences in the levels of serum CRP(45.24±2.01 mg/L,19.02±3.27 mg/L and 3.26±0.77 mg/L),PCT(1.58±0.52μg/L,0.82±0.16μg/L and 0.31±0.09μg/L),IL-8(42.77±8.84μg/L,26.35±5.91μg/L and 13.02±3.79μg/L),TNF-α(9.22±2.61μg/L,5.02±1.49μg/L and 3.32±0.96μg/L)in RMPP group,common pneumonia group and control group(F=4207.164,457.187,410.300,214.875,all P<0.05).Pearson correlation analysis showed that the expression levels of serum LncRNA MALAT1 and LncRNA NNT-AS1 were posit

关 键 词：难治性肺炎支原体肺炎 长链非编码核糖核酸 肺腺癌转移相关转录因子1 烟酰胺核苷酸反义转氢酶RNA1 

分 类 号：R563.15[医药卫生—呼吸系统] R392.11[医药卫生—内科学]