急性冠脉综合征患者血清LncRNA XIST和miR-330-3p水平表达与心脏自主神经功能的相关性研究  

Correlation between the Expression of Serum LncRNA XIST and miR-330-3p Levels and Cardiac Autonomic Nerve Function in Patients with Acute Coronary Syndrome

作　　者：刘英华[1] 王玉霞[1] 卢海英[2] 曾娟[2] 刘亚宁[1] 高晓丽[1] 王静[3] LIU Ying-hua;WANG Yu-xia;LU Hai-ying;ZENG Juan;LIU Ya-ning;GAO Xiao-li;WANG Jing(Department of Internal Medicine-Cardiovascular,Huabei Petroleum Administration Bureau General Hospital,Hebei Renqiu 062552,China;Department of Functional,Huabei Petroleum Administration Bureau General Hospital,Hebei Renqiu 062552,China;Department of Clinical Laboratory,Huabei Petroleum Administration Bureau General Hospital,Hebei Renqiu 062552,China)

机构地区：[1]华北石油管理局总医院心内科,河北任丘062552 [2]华北石油管理局总医院功能科,河北任丘062552 [3]华北石油管理局总医院检验科,河北任丘062552

出　　处：《现代检验医学杂志》2022年第4期64-68,204,共6页Journal of Modern Laboratory Medicine

基　　金：河北省医学科学研究课题计划项目(编号20201228)。

摘　　要：目的检测急性冠脉综合征(acute coronary syndrome,ACS)患者血清长链非编码RNA X染色体失活特异转录物(LncRNA X inactivate-specific transcript,LncRNA XIST)和微小RNA(miR)-330-3p水平,分析二者与心脏自主神经功能的关系。方法选取2020年2月~2021年6月在华北石油管理局总医院诊断为ACS的患者156例,包括不稳定型心绞痛(unstable angina,UA)80例(UA组)和急性心肌梗死(acute myocardial infarction,AMI)76例(AMI组);另选取同期体检健康者100例作为对照组。实时荧光定量PCR法检测受试者血清LncRNA XIST和miR-330-3p水平;24 h动态心电图检测受试者心率变异性(heart rate variability,HRV)时域指标[包括:24 h内正常R-R间期标准差(SDNN)、每5 min R-R间期平均值的标准差(SDANN)、每5 min R-R间期标准差的平均值(SDNN index)、相邻R-R间期之差的均方根(RMSSD)及相邻R-R间期相差>50 ms心搏数占总心搏数的百分比(PNN50)]以及Pearson法分析ACS患者血清LncRNA XIST和miR-330-3p水平与HRV时域指标的相关性。结果与对照组比较,UA组和AMI组血清LncRNA XIST(1.37±0.26,1.69±0.31 vs 1.09±0.22)水平升高(F=113.587,P=0.000),miR-330-3p(0.82±0.15,0.53±0.12 vs 1.03±0.18)及SDANN(115.96±19.57 ms,98.96±16.57 ms vs 133.43±21.01 ms),SDNN(113.84±15.26 ms,101.84±11.22 ms vs 136.41±31.53 ms),SDNN index(54.68±6.72 ms,46.73±5.14 ms vs 64.68±10.64 ms),RMSSD(27.48±5.62 ms,21.37±3.64 ms vs 36.48±8.62 ms)和PNN50(7.58%±2.83%,4.18%±1.13%vs 10.72%±3.21%)水平均降低(F=54.923~225.201,均P=0.000),差异均有统计学意义。与UA组比较,AMI组血清LncRNA XIST水平升高(t=10.792,P<0.05),miR-330-3p及SDANN,SDNN,SDNN index,RMSSD和PNN50水平降低,差异有统计学意义(t=4.743~16.538,均P<0.05);ACS患者血清LncRNA XIST与SDNN index,RMSSD和PNN50呈负相关(r=-0.465,-0.532,-0.542,均P<0.05);miR-330-3p与SDNN index,RMSSD和PNN50呈正相关(r=0.471,0.562,0.561,均P<0.05)。结论ACS患者血清LncRNA XIST水平升高,miR-330-3p水平降低,与心脏自主Objective To detect the levels of long non-coding RNA X inactivate-specific transcript(LncRNA XIST)and microRNA-330-3p(miR-330-3p)in the serum of patients with acute coronary syndrome(ACS),and their relationship with cardiac autonomic nerve function was analyzed.Methods A total of 156 patients diagnosed with ACS in General Hospital of North China Petroleum Administration from February 2020 to June 2021 were selected,including 80 patients with unstable angina(UA group)and 76 patients with acute myocardial infarction(AMI group).Another 100 healthy subjects were selected as the control group.Real-time fluorescent quantitative PCR was used to detect the levels of LncRNA XIST and miR-330-3p in the serum of subjects.24 h ambulatory electrocardiogram was used to detect the subject’s heart rate variability(HRV)time domain indicators[include:standard deviation of normal R-R interval within 24 h(SDNN),the standard deviation of the mean value of R-R interval every 5 min(SDANN),the mean of standard deviation of R-R interval every 5 min(SDNN index),the root mean square of the difference between adjacent R-R intervals(RMSSD),and the percentage of heart beats with a difference of>50 ms between adjacent R-R intervals to the total heart rate(PNN50)].Pearson method was used to analyze the correlation between LncRNA XIST and miR-330-3p levels in serum of ACS patients and HRV time domain indicators.Results Compared with the control group,the serum LncRNA XIST(1.37±0.26,1.69±0.31 vs 1.09±0.22)level of UA group and AMI group increased(F=113.587,P=0.000),the miR-330-3p(0.82±0.15,0.53±0.12 vs 1.03±0.18)and SDANN(115.96±19.57 ms,98.96±16.57 ms vs 133.43±21.01 ms),SDNN(113.84±15.26 ms,101.84±11.22 ms vs 136.41±31.53 ms),SDNN index(54.68±6.72 ms,46.73±5.14 ms vs 64.68±10.64 ms),RMSSD(27.48±5.62 ms,21.37±3.64 ms vs 36.48±8.62 ms)and PNN50(7.58%±2.83%,4.18%±1.13%vs 10.72%±3.21%)levels decreased(F=54.923~225.201,all P=0.000),the differences were statistically significant,respectively.Compared with the UA group,serum Lnc

关 键 词：急性冠脉综合征 长链非编码RNA X染色体失活特异转录物 微小核糖核酸-330-3p 心率变异性时域指标 

分 类 号：R541.4[医药卫生—心血管疾病] R392.11[医药卫生—内科学]