Recent biomedical advances enabled by HaloTag technology  

作　　者：WEIYU CHEN MUHSIN HYOUNIS ZHONGKUO ZHAO WEIBO CAI 

机构地区：[1]The Fourth Affiliated Hospital,Zhejiang University School of Medicine,Yiwu,322000,China [2]International Institutes of Medicine,The Fourth Affiliated Hospital of Zhejiang University School of Medicine,Yiwu,322000,China [3]Departments of Radiology and Medical Physics,University of Wisconsin—Madison,Madison,WI,53705,USA

出　　处：《BIOCELL》2022年第8期1789-1801,共13页生物细胞（英文）

基　　金：The authors are grateful for financial support from the University of Wisconsin—Madison and National Institutes of Health(P30CA014520).

摘　　要：The knowledge of interactions among functional proteins helps researchers understand disease mechanisms and design potential strategies for treatment.As a general approach,the fluorescent and affinity tags were employed for exploring this field by labeling the Protein of Interest(POI).However,the autofluorescence and weak binding strength significantly reduce the accuracy and specificity of these tags.Conversely,HaloTag,a novel self-labeling enzyme(SLE)tag,could quickly form a covalent bond with its ligand,enabling fast and specific labeling of POI.These desirable features greatly increase the accuracy and specificity,making the HaloTag a valuable system for various applications ranging from imaging to immobilization of POI.Notably,the HaloTag technique has already been successfully employed in a series of studies with excellent efficiency.In this review,we summarize the development of HaloTag and recent advanced investigations associated with HaloTag,including in vitro imaging(e.g.,POI imaging,cellular condition monitoring,microorganism imaging,system development),in vivo imaging,biomolecule immobilization(e.g.,POI collection,protein/nuclear acid interaction and protein structure analysis),targeted degradation(e.g.,L-AdPROM),and more.We also present a systematic discussion regarding the future direction and challenges of the HaloTag technique.

关 键 词：HALOTAG Site-specific labeling Biomolecule interaction Molecular imaging Positron emission tomography 

分 类 号：Q50[生物学—生物化学]